槟榔提取物F57对大鼠结肠平滑肌运动的影响  

Study of Areca Nut Extract F57 on Contractile Activity of Rat Colonic Smooth Muscle Strips in Vitro

作　　者：范尧夫[1] 胡兵[2] 李晨[2] 魏睦新[1] 

机构地区：[1]南京医科大学第一附属医院中医科,南京210029 [2]南京中医药大学附属徐州市中医院消化内科,徐州221000

出　　处：《江西中医学院学报》2011年第6期74-76,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：江苏省自然科学基金课题(SBK200920943);江苏省中医药管理局课题(LZ09108)

摘　　要：目的:观察从中药槟榔中分离出的F57对离体大鼠结肠平滑肌运动的影响。方法:制备大鼠结肠平滑肌肌条,以0.9%氯化钠溶液(NS)和乙酰胆碱(Ach)作对照,观察不同浓度的F57对离体大鼠平滑肌肌条的收缩效应。结果:与NS组比较,不同浓度的F57对离体大鼠结肠平滑肌均有不同程度的促进作用,差异具有统计学意义(P﹤0.05)。以Ach标化实验数据,0.1 g/L的F57组与1×10-4 mol/L的Ach对离体大鼠结肠平滑肌的收缩效应无明显差异;当F57的浓度增大到1 g/L时候,肌条收缩效应基本达到峰值,收缩不再随着浓度的增大而增加。结论:从槟榔中分离出的F57对大鼠结肠平滑肌有明显促进收缩的作用,且收缩的效应与剂量呈正相关,可以进一步对F57进行分离单体的研究。Objective：To study the effect of Areca nut extract F57 on the contractile activity of rat colonic smooth muscle strips in vitro.Methods：The colonic longitudinal smooth muscle strips of rats were prepared and 0.9%saline and acetylcholine were used as control.We observed the effects of Areca nut extract F57 on the spontaneous contraction of the strips.Results：The different concentrations of Areca nut extract F57 promoted the spontaneous contraction of colonic muscle strips in different degrees compared with the saline group（P0.05）.The result of experiment was standardized by Ach.The peak contraction amplitude and the area under the curve of contraction of colonic smooth muscle strips were not significantly different between Areca nut extract F57 at a concentration of 0.01g/L and Ach at a concentration of 1×10-4mol/L;When the concentration of F57 increased to 1g/L,the contraction amplitude and the area under the curve of contraction of colonic smooth muscle strips would reach the peak.Conclusion：Areca nut extract F57 shows stimulative effects on spontaneous contraction of rat colonic smooth muscle strips in vitro and has positively correlated with the dose.We can make further study about F57

关 键 词：槟榔提取物 平滑肌 大鼠 

分 类 号：R284.2[医药卫生—中药学]