嫁接对西瓜根际微生物种群数量及群落结构的影响  

Effects of Grafting on Rhizosphere Microorganisms Quantity and Community Structure of Watermelon

作　　者：雷娟利[1] 寿伟松[1] 董文其[1] 张成浩[1] 徐志豪[1] 周艳虹[2] 喻景权[2] 

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021 [2]浙江大学园艺系,浙江杭州310029

出　　处：《中国蔬菜》2011年第2期62-66,共5页China Vegetables

基　　金：国家科技支撑计划(2008BADA6B02);浙江省自然科学基金(Y3100003)

摘　　要：分别采用微生物培养的方法、16SrDNA PCR-RFLP方法和phlD基因PCR-RFLP方法研究了嫁接对西瓜根际微生物种群数量、细菌群落结构及拮抗菌(2,4-DAPG产生菌)群落结构的影响。结果表明:①嫁接西瓜根际细菌和真菌数量有所提高,而放线菌数量则有所减少。②嫁接西瓜与自根西瓜具有1个相同的主要根际细菌基因型;不同砧木嫁接的西瓜与自根西瓜也分别具有不同基因型的细菌。③嫁接西瓜具有与自根西瓜不同的主要根际拮抗菌基因型。The changes of microorganisms population,bacterial community structure and 2,4-DAPG producers community structure in rhizosphere soil of grafted watermelon〔Citrullus lanatus（Thunb.） Matsum.et Nakai〕plants were studied using the methods of microorganism culture,16S rDNA PCR-RFLP and phl D gene PCR-RFLP,respectively.The results showed that the bacterial and fungal population in rhizosphere soil of grafted watermelon was increased and that of the actinomyces population was decreased;the grafted watermelon had one similar main 16S rDNA genotype in rhizosphere soil with the non-grafted watermelon,although the grafted watermelon with different stock had their unique 16S rDNA genotypes different from non-grafted watermelon;the grafted watermelon had different main 2,4-DAPG producers phl D genotypes from non-grafted watermelon.

关 键 词：西瓜嫁接 根际微生物 细菌 群落结构 2 4-DAPG产生菌 

分 类 号：S651[农业科学—果树学]