Wnt信号分子在神经管缺陷(NTD)发生过程中的表达及其可能的作用机制  

Expression and possible molecular mechanism of Wnt signaling in the process of neural tube defect

作　　者：张建[1] 陈穗[1] 罗学胜[1] 杨忠[2] 李红丽[2] 蔡文琴[2] 

机构地区：[1]宁波市解放军第113医院干部病房,宁波315040 [2]重庆市第三军医大学组织学与胚胎学教研室,重庆400038

出　　处：《中国组织化学与细胞化学杂志》2011年第6期572-577,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：第三军医大学回国人员启动基金

摘　　要：目的探讨Wnt信号分子在神经胚形成和神经管缺陷(NTD)发生过程中的作用及其可能的分子调控机制。方法用Western blot方法半定量检测正常及NTD小鼠胚胎的脑泡及脊髓神经组织中Wnt信号分子的变化。采用流式细胞技术检测正常和神经管缺陷(NTD)胚胎脑泡及脊髓神经组织神经上皮细胞周期动力学的变化。结果与正常胚胎的脑泡及脊髓神经组织比较,NTD胚胎的脑泡及脊髓神经组织的β-catenin蛋白表达量明显减弱;而GSK-3β蛋白表达量明显增强。流式细胞仪的检测结果显示:与正常E10.5d胚胎脑泡及脊髓神经组织的神经上皮相比,神经管缺陷模型胚胎的脑泡及脊髓神经组织的神经上皮细胞处于G0/G1期的细胞百分比明显增高,而神经上皮细胞处于S期的细胞百分比则明显降低。结论神经管缺陷的发生与Wnt信号途径的变化是密切相关的,Wnt信号分子的变化可能正是神经管缺陷形成中细胞增殖抑制的相关分子机制。神经管上皮细胞的增殖抑制及凋亡可能是NTD发生的重要细胞基础。Objective To investigate the role and the possible molecular mechanism of Wnt signaling in the process of neural tube defect（NTD）.Methods Western blot was performed to detect the expression of Wnt signaling in the normal and NTD brain and spinal nerve tissue.By using flow cytometry,the cell dynamic changes of Wnt signaling in the normal and NTD brain and spinal nerve tissue was detected.Results In the NTD brain and spinal nerve tissue,the level of β-catenin decreased and GSK-3β increased significantly as compared with those of the control groups.Flow cytometry showed that a higher proportion of G1 phase cells existed in NTD mice than in the normal group at E10.5（P0.01）.and the percentage of S phase cells showed a significant decrease in the meantime.Conclusion Our results suggest that the wnt/β-catenin pathway may be involved in the process of NTD as well.The decreased β-catenin level caused by inhibition of this pathway may act as a molecular mechanism in the NTD process.

关 键 词：Wnt信号分子 神经管缺陷 WESTERN BLOT 流式细胞技术 小鼠 

分 类 号：R321.6[医药卫生—人体解剖和组织胚胎学]