痰液肺癌细胞Ras基因和P63基因的表达与分子生物学意义  

Molecular biological observation and study of lung cancer cells from sputum

作　　者：许立莉[1,2] 李淼[3] 杜明伟[1,2] 陈江帆[1,2] 李建华[1,2] 

机构地区：[1]中国医科大学附属第一医院病理科 [2]中国医科大学病理教研室,沈阳1100012 [3]辽宁中医药大学附属第二医院病理科,沈阳110034

出　　处：《中国组织化学与细胞化学杂志》2011年第6期616-622,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(30801131);教育部高等学校博士学科点专项科研基金(200804861045)

摘　　要：目的研究骨髓间充质干细胞分化为心肌细胞过程中Notch表达的研究。方法别用免疫细胞化学方法和Western-blot方法检测167例患者痰液中Ras基因和P63基因的表达情况,分析其对早期肺癌诊断的意义。结果肺癌患者痰液中的Ras基因在痰脱落细胞中有明显高表达,阳性率为75%。P63基因在肺癌痰细胞中阳性表达率为34.78%。结论利用Ras基因标记物可以标记出痰液中的肺非典型增生细胞和肺癌细胞,有利于肺癌的早期发现和诊断,并且可以作为肺癌的普查指标或手段。Objective To investigate the value of the Ras gene in sputum cells in the diagnosis of lung cancer,and the diagnostic value of combination detection of Ras and P63 genes in lung cancer.Methods The expression of Ras and P63 genes in sputum was detected by immuncytochemistry and Western-blot method,and its meaning for the early diagnosis of lung cancer was analyzed.Results 75% lung cancer cells showed Ras gene mutation,much higher than in normal cells（P0.05）.34.78% lung cancer cells showed P63 gene mutation,also higher than in normal sputum cell（P0.05）.Conclusion The Ras gene can be used to label suspicious and early cancerous cells in the sputums for the early diagnosis of lung cancer.

关 键 词：肺癌 RAS基因 P63基因 痰 液基 

分 类 号：R349.5[医药卫生—基础医学]