抗菌肽对蛋鸡血清免疫指标及脾脏白细胞介素2 mRNA表达量的影响  

Effects of Antimicrobial Peptides on Serum Immune Indices and IL-2 mRNA Expression in Spleen of Laying Hens

作　　者：吕尊周[1] 袁肖笑[1] 蔡兆伟[1] 尹兆正[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029

出　　处：《动物营养学报》2011年第12期2183-2189,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：浙江省重大科技专项(2008C12075-2)

摘　　要：本试验旨在研究饲粮不同水平抗菌肽对蛋鸡血清免疫指标及脾脏白细胞介素2(IL-2)mRNA表达量的影响。选用480只30周龄海赛克斯褐壳蛋鸡,随机分成4组,每组4个重复,每个重复30只鸡。对照组(Ⅰ组)饲喂基础饲粮,试验组分别在基础饲粮中添加200(Ⅱ组)、400(Ⅲ组)、600 mg/kg(Ⅳ组)抗菌肽,试验期4周。结果表明,饲粮中添加不同水平抗菌肽可不同程度地提高脾脏系数,其中Ⅲ组显著高于对照组(P<0.05)。与对照组相比,试验组血清免疫球蛋白A含量分别提高了7.69%(P>0.05)、19.40%(P<0.05)和14.38%(P>0.05),血清免疫球蛋白G含量分别提高了12.69%(P>0.05)、21.24%(P<0.05)和9.10%(P>0.05),而抗菌肽对血清免疫球蛋白M、补体3和补体4含量影响不显著(P>0.05)。Ⅱ组和Ⅲ组中脾脏IL-2 mRNA的表达量均显著高于Ⅰ组和Ⅳ组(P<0.05),Ⅳ组与Ⅰ组之间差异不显著(P>0.05)。由此可见,抗菌肽能提高蛋鸡的免疫功能,且饲粮中添加400 mg/kg抗菌肽效果最佳。The objective of this experiment was to study the effects of different dietary levels of antimicrobial peptides （AMPs） on serum immune indices and IL-2 mRNA expression in spleen of laying hens. Four hun- dred and eighty 30-week-old Hisex hens were randomly divided into four groups with four replicates per group and thirty hens per replicate. Hens in control group （ group I ） were fed a basal diet, and the others in experi- mental groups （groups II , 11 and 1V ） were fed the basal diet supplemented with 200, 400 and 600 mg/kg AMPs, respectively. The experiment lasted for four weeks. The results showed as follows： dietary AMPs in experimental group could increase spleen coefficient, the spleen coefficient in group l]I was significantly higher than that in control group （ P 〈 0.05 ）. Compared with the control group, the content of serum immunoglobu- lin A in groups II ,]lI and IV was increased by 7.69% （P〉0.05）, 19.40% （P 〈0.05）and 14.38% （P〉 0.05）, respectively, and serum immunoglobulin G content was increased by 12.69% （P 〉 0.05）, 21.24% （P 〈0.05） and 9.10% （P 〉0.05）, respectively. However, there were no significant effects on the contents of serum immunoglobulin M, complement 3 and complement 4. The expression level of IL-2 mRNA in spleen in groups lI and 111 was significantly higher than that in groups I and IV （ P 〈 0.05）, but there was no sig- nificant difference between group I and group IV （ P 〉 0.05 ）. In conclusion, AMPs can enhance immune function of laying has, and adding 400 mg/kg AMPs to diets for laying hens has the optimum effect.

关 键 词：抗菌肽 蛋鸡 免疫功能 IL-2MRNA 

分 类 号：S816.7[农业科学—饲料科学] S831[农业科学—畜牧学]