熟地强筋合剂对大鼠骨髓基质细胞向成骨细胞分化的影响  

The Effect on Differentiation to Osteoblast of SD Rat'S Bone Marrow Stromal Cells in vitro by Shu Di Qiang Jin Mixture

作　　者：孙克民[1] 王平[1] 卢启贵[1] 黄东红[1] 裴代平[1] 

机构地区：[1]广东省深圳平乐骨伤科医院,深圳518010

出　　处：《中国医药导刊》2011年第11期1938-1939,共2页Chinese Journal of Medicinal Guide

摘　　要：目的:观察熟地强筋合剂在骨髓基质细胞向成骨分化过程中的作用。方法:采用体外培养大鼠骨髓基质细胞,分别观察各含药血清组碱性磷酸酶染色、Ⅰ型胶原染色情况;取各组不同时间点培养液测定碱性磷酸酶比活性及骨钙素含量。结果:碱性磷酸酶染色强弱顺序为D组>C组>B组,Ⅰ型胶原染色强弱顺序为D组>C组>B组>A组,碱性磷酸酶比活性各加药组与对照组比较明显提高(P<0.05);各加药组与对照组比较BGP含量均明显提高。结论:熟地强筋合剂能促进大鼠骨髓基质细胞增殖,诱导大鼠骨髓基质细胞向成骨细胞分化。Objective：To observe the differentiation to osteoblast of SD rat＇ s marrow stromal cell cultured by Shu Di Qiang Jin Mixture.Methods：The serum pharmacology method was used to culture the SD rat＇ s marrow stromal cell.The alizarin Bordeaux starting,Van-GiegonⅠcollagen staining method were used to observe the expressions of ALP and typeⅠcollagen respectively on different collection time.Results：The intensity of ALP dyeing sequenced as：DCB,and collagenⅠdyeing sequenced as：DCBA.The alkaline phosphatase activity and the amount of bone glaprotein were higher in B,C,D groups compared with the control group at the same time（P0,05）.Conclusion：Shu Di Qiang Jin Mixture could accelerate the proliferation of the BMSCs,and could induce the BMSCs differentiation,accelerate the proliferation and differentiation of the BMSCs.

关 键 词：熟地强筋合剂 骨髓基质细胞 成骨细胞 诱导分化 

分 类 号：R285.5[医药卫生—中药学]