GSTM1基因多态性与抗结核药物所致肝损害相关性研究  

Association of GSTM1 Genetic Polymorphisms with Susceptibility to Antituberculosis Drug Induced Hepatotoxicity,A Study in Chinese Population

作　　者：王涛[1] 韩旭 王仲元[1] 蒋滢[3] 刘琳[1] 安慧茹[1] 贺路星[1] 

机构地区：[1]解放军309医院结核病研究所,北京100091 [2]北京市总政治部军事监狱门诊内科,北京100012 [3]解放军309医院检验科,北京100091

出　　处：《中国医药导刊》2011年第12期2164-2165,2158,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:检测谷胱甘肽巯基转移酶M1(GSTM1)基因多态性与抗结核药物性肝损害(ADIH)的相关性,探讨抗结核药物肝损害的遗传基础及易感因素。方法:收集本院104例抗结核药物性肝损害患者及111例对照外周血液标本,采用聚合酶链反应(PCR)及聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)测定GSTM1各基因型分布频率,结合临床资料,采用x^2检验及Logistic回归分析GSTM1基因多态性与抗结核药物肝损害发病风险相关性。结果:GSTM1(null)基因型与GSTM1(present)基因型相比,抗结核药物肝损害发病风险呈增高倾向,但无统计学差异(OR=1.566,95%CI=0.895-2.741,P=0.116)。结论:无效基因型GSTM1(null)抗结核药物肝损害发病风险呈增高倾向,但无统计学差异,需进一步扩大样本研究确认。Objective：Tbis study was aimed to investigate the association of genetic polymorphism of glutathione S-transferase Ml（GSTM1） with the susceptibility to ADM in Chinese tuberculosis patients.Methods：All patients were treated with the combination of isomazid,rifampicin,pyrazinamide and ethambufol.Genomic DNA from 104 tuberculosis patients with ADIH 1104 paptients） and without ADIH（111 patients） were analyzed for the distribution frequencies of CYP2E1 Rssl and GSTM1 Rsal genotypes by polymerase chain reaction（PCR） and polymerase chain reaction-restriction fragment length polyimorpKism（PCR-RFLP）.The association of genetic polymorphism of GSTM1 with the susceptibility to ADIH was calculated usingχ~2 test and logistic regression analysis.Results：Compared with the GSTM1（present） genotype,the GSTM1（null） genotype tended to increase the susceptibility to ADIH.however,the association with ADIH was not significant（OR=1.566,95%CI=0.895-2.74l,P=0.116）.Conclusions：Our results indicated that the tendency of GSTMI （null） genotype increasing the susceptibility to ADIH.

关 键 词：GSTM1 基因多态性 抗结核药物性肝损害 

分 类 号：R595.3[医药卫生—内科学]