组织脱细胞方法的研究进展  

Progress on Research of Decellularization Methods

作　　者：孙亨[1] 王艳霞[2] 吴江[1] 

机构地区：[1]四川大学华西基础医学与法医学院,成都610041 [2]四川大学图书馆,成都610041

出　　处：《四川解剖学杂志》2011年第4期24-30,共7页Sichuan Journal of Anatomy

基　　金：国家自然科学基金项目资助课题(10802054)

摘　　要：脱细胞组织和器官作为一种生物材料,已经在组织工程和再生医学中成功应用,各种组织的脱细胞方法也受到关注。组织脱细胞的方法可以分为物理、化学和酶学方法,各组织脱细胞的效率和结果与组织的来源、结构和组成、脱细胞的方法等都有关,不同的方法对不同的组织不仅清除细胞的效果不同,对细胞外基质(ECM)的影响也不同,这反过来又会使宿主对移植的脱细胞材料产生不同的反应。因此,我们要根据组织特点和不同脱细胞方法的特点来选择最优的脱细胞方法,以期达到理想的效果。本文介绍了最常用的一些脱细胞方法,包括物理、化学和酶学方法,并简单描述了它们对组织支架的影响。Decellularized tissues and organs have been successfully used in tissue engineering and regenerative medicine applications as biologic materials,and the decellularization methods have received much attention.Decellularization methods can be classified to physical,chemical and enzymatic methods.The efficiency of decellularization depends on the origin,components and architecture of the tissue,and the method we used.Different methods affect not only the efficiency of cell removal,but also the components,ultrastructure and mechanical behavior of extracellular matrix（ECM）,which in turn affects the host response to the implanted decellularized materials.Therefore,we need to choose the optimized method according to the characteristics of the tissue of interest.In this article,some most commonly used decellularization methods are introduced,including physical,chemical and enzymatic methods,and described the impacts to the tissue scaffolds briefly.

关 键 词：脱细胞 细胞外基质 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]