日本血吸虫SJCHGC01894分子T细胞表位肽的预测及免疫原性鉴定  

PREDICTION AND IDENTIFICATION OF T CELL EPITOPE WITHIN PROTEIN SJCHGC01894 FROM SCHISTOSOMA JAPONICUM

作　　者：赵犇鹏[1,2] 贾侃[1,2] 张艳丽[2] 李莹[1,2] 林矫矫[2] 李利珍[1] 冯新港[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2011年第3期43-48,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点基础研究发展计划(973)课题(2007CB513108)

摘　　要：利用服务器SYFPEITHI、HLAPRED、MHCPred预测日本血吸虫SJCHGC01894分子的T细胞表位,采用ClusPro服务器将序列表位和MHCⅡ分子进行空间模拟结合分析,并用四分臂偶联到多聚赖氨酸骨架上的方法合成多肽,然后用CCK8法鉴定表位肽刺激小鼠淋巴细胞的增殖效果,通过流式细胞术测定免疫小鼠CD4+T细胞内细胞因子的分泌情况。检测结果表明,人工合成的表位肽P1(NH-MSSLSKAEIEDIREV-COOH)可促进免疫鼠脾淋巴细胞增殖,P1刺激后免疫鼠CD4+T细胞中分泌的IFNγ/IL-4数值上升。本研究初步鉴定了SJCHGC01894分子中的一个潜在的Th1细胞表位,为合理筛选抗血吸虫保护性抗原或表位提供了依据。To predict and identify T cell epitopes of SJCHGC01894 molecule from Schistosoma japonicum,Th cell epitopes were predicted using SYFPEITHI,HLAPRED and MHCPred servers,subsequently.The peptides were selected for further structure-based analysis using the ClusPro program.The candidate peptide was synthesized artificially（coupled with the poly-lysine skeleton）.The proliferation of spleen lymphocytes in mice vaccinated with candidate peptide was assessed by using CCK8（Cell Counting Kit-8） method.Intracellular cytokines of CD4＋T cell were measured by flow cytometry.The results showed that the predicted peptide P1 could stimulate the lymphocytes proliferation of the immunized mice,and the ratios of the IFN-γ-producing CD4＋ cells/IL-4-producing CD4＋ cells increased following immunization and in vitro stimulation with the peptide P1.One potential Th1 epitope within SJCHGC01894 molecule was confirmed using in silico prediction and experimental validation,and provided the experimental basis for further screenings of protective T cell antigens/epiotopes against Schistosoma japonicum.

关 键 词：日本血吸虫 SJCHGC01894 T细胞表位 预测 免疫原性 

分 类 号：S852.735.243[农业科学—基础兽医学]