PPAR-γ pro12ala基因多态性与甲状腺相关性眼病的相关性研究  

The relationship between PPAR-γ pro12ala gene polymorphism and thyroid associated ophthalmopathy

作　　者：闫春芳[1] 孙斌[2] 尹晓艳[1] 

机构地区：[1]山西医科大学研究生院眼科学系,山西太原030001 [2]山西医科大学第一医院眼科,山西太原030001

出　　处：《中国当代医药》2012年第11期10-12,共3页China Modern Medicine

摘　　要：目的通过研究过氧化物酶体增殖激活受体-γ(PPAR-γ)pro12ala位点基因多态性在山西地区部分汉族人中的分布规律及其与甲状腺相关性眼病(TAO)的关系,从而探讨TAO可能的遗传因素及发病机制,为TAO的防治提供一定的实验依据。方法研究对象共有166例,共分为3组,其中TAO患者51例,Graves病(GD)患者55例及正常对照者60例,分别选取研究对象的外周抗凝血来提取基因组DNA,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)的研究方法,来检测研究对象PPAR-γ pro12ala位点基因型和等位基因的频率。结果 G等位基因TAO组频率明显高于GD组与正常对照组(27.8%vs13.6%,13.3%,P<0.05);CG+GG基因型的频率TAO患者组明显高于GD患者组与正常对照组(52.9%vs25.5%,26.7%,P<0.05),GD组及正常对照组的基因频率的差异无统计学意义。结论山西地区部分汉族人群中存在PPAR-γpro12ala位点基因多态性;PPAR-γ pro12ala位点基因多态性可能与山西地区部分汉族人TAO的发病相关。Objective Through the studied of peroxisome proliferator-activated receptor-γ（PPAR-gamma） pro12ala polymorphism in Chinese Han people in Shanxi Province,so as to explored the genetic factors and the pathogenesis of TAO may provide some experimental evidence for the prevention and treatment of TAO.Methods Subjects a total of 166 cases divided into three groups,of which 51 cases of TAO patients,55 patients of Graves＇ disease（GD） and normal controls were 60 cases.The research object were selected to extract DNA genome from the peripheral anticoagulant,the polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） research methods was used,to detect ppar-gamma pro12ala site genotype and allele frequency of the research object.Results The frequency of G allele TAO group was significantly higher than the GD group and normal control group（27.8% vs 13.6%,13.3%,P 0.05）;CG ＋ GG genotype frequency of TAO patients were significantly higher than in patients with GD and normal controls group（52.9% vs 25.5%,26.7%,P 0.05）.GD group and the normal control group of gene frequency difference wasn＇t statistically significant.Conclusion There is PPAR-γ gene polymorphism pro12ala in Chinese Han people in Shanxi Province;PPAR-γ gene polymorphism pro12ala may be associated with the incidence of TAO in Chinese Han people in Shanxi Province.

关 键 词：PPAR-Γ pro12ala位点 GRAVES病 TAO 基因多态性 

分 类 号：R581[医药卫生—内分泌]