枸杞多糖对肝癌荷瘤小鼠的抗肿瘤作用及其机制  

Antitumor Activity of Lycium Barbarum Polysaccharide (LBP) in Hca-F Tumor-bearing Mice and its Mechanism

作　　者：蒋艳[1,2] 姜孝新[1] 

机构地区：[1]南华大学附属第一医院,湖南衡阳421001 [2]南华大学药物药理研究所,湖南衡阳421001

出　　处：《肿瘤药学》2011年第4期391-394,共4页Anti-Tumor Pharmacy

摘　　要：目的研究枸杞多糖(LBP)对小鼠Hca-F肝癌的生长抑制作用及机制。方法建立小鼠Hca-F肝癌模型,腹腔注射不同浓度的枸杞多糖连续10d。称重检测枸杞多糖对肿瘤生长、脾脏和胸腺指数的影响;采用MTT法、ELISA法分析枸杞多糖对荷瘤小鼠脾巨噬细胞的吞噬活性、细胞因子分泌等免疫指标的影响。结果枸杞多糖对小鼠实体瘤生长具有抑制作用,枸杞多糖40mg·kg-1与对照组相比有显著差异(P<0.05);实验组小鼠脾指数和胸腺指数均高于对照组。枸杞多糖40mg·kg-1即可明显促进IL-2的产生,降低VEGF蛋白的表达。结论枸杞多糖对Hca-F肝癌小鼠肿瘤生长有抑制作用,可能与提高荷瘤小鼠细胞和分子免疫活性有关。Objective To investigate the antitumor activity of Lycium barbarum polysaccharide （LBP） in Hca-F tumor-bearing mice and its mechanism.Method The established Hca-F tumor model mice were intraperitoneally injected with different concentration of LBP for 10 d.Effect of LBP on tumor,spleen and thymus of Hca-F tumor-bearing mice were detected after treatment.The phagocytic activity of macrophages was assessed by MTT assays,and the immune organic index like contents of IL-2 and VEGF were detected by ELISA method.Results LBP could obviously inhibit growth of Hca-F carcinoma at dose of 40 mg·kg^-1 for 10 days （P〈0.05）.The indices of spleen and thymus in test group were higher than that in control.And the phagocytosis activity of spleenocyte and production of IL-2 were promoted by LBP in test groups,whereas the protein expression of VEGF was decreased in test groups.Conclusion LBP might inhibit the growth of Hca-F tumor cells through improving the immune function of immunocytes and cytokines in mice.

关 键 词：枸杞多糖 HCA-F 抗肿瘤 免疫 

分 类 号：R979[医药卫生—药品]