转化生长因子β1在糖尿病大鼠肾脏表达中的研究  

Expression changes of transforming growth factor-β1 in kidney of streptozotocin-induced diabetic rats

作　　者：叶小林 

机构地区：[1]浙江省桐庐县第一人民医院内分泌科,浙江桐庐311500

出　　处：《中国现代医生》2012年第11期8-9,共2页China Modern Doctor

基　　金：浙江省桐庐县科学技术局2008年(第一批)科技项目(2008C165)

摘　　要：目的探讨链脲佐菌素(streptozotocin,STZ)诱导的糖尿病大鼠转化生长因子β1(Transforming growth factor-β1,TGF-β1)的表达情况。方法实验采用成年雄性Sprague-Dawle大鼠20只,随机分为两组各10只,糖尿病组尾静脉注射STZ制成糖尿病模型,对照组尾静脉注射枸橼酸钠缓冲液。两组大鼠4周后处死,取肾脏组织行苏木精伊红染色法观察糖尿病肾脏组织的形态变化,同时行免疫组织化学SABC半定量检测TGF-β1蛋白的表达水平。结果糖尿病组肾脏组织光镜下出现肾小球的毛细血管球肥大,肾小囊腔呈裂隙状,基底膜轻度增厚,系膜增生,肾小管上皮细胞显示空泡和颗粒变性。TGF-β1蛋白在糖尿病大鼠肾脏上皮细胞和肾小球系膜细胞表达显著增高(P<0.01)。结论实验动物的高血糖状态会损害肾脏组织,高血糖可能通过活化大鼠肾脏组织内TGF-β1,从而促进肾间质结缔组织增生和纤维化,从而导致肾小球硬化而引起糖尿病肾病。Objective To explore the expression pattern of transforming growth factor-β1（TGF-β1）in kidney of streptozotocin（STZ）-induced diabetic rats.Methods We employed 10 STZ-induced male adult Sprague-Dawle diabetic rats as diabetic group rat and 10 normal rats as control group,to study the alteration of the TGF-β1expressions in the kidney by immunohistochemistry staining.Ultrastructure changes of kidney were determined by hematoxylin eosin staining（H-E staining）.Results After the induction four weeks,the diabetic kidney tissue showed glomerules hypertrophy,renal tubular epithelial cell granular degenerationand and hyperplasia of glomerular basement membrane.All of which were characteristic pathological changes of diabetic nephropathy.A significant increase in TGF-β1 expression were also observed in the diabetic rat＇s kidney（P〈0.01）.Conclusion Our study provides experimental evidence that hyperglycemia could damage kidney.And the altered expression of TGF-β1 may contribute to glomerular sclerosis and leades to diabetic nephropathy by renal interstitium accrementition and fibrous degeneration under hyperglycemia.

关 键 词：糖尿病肾病 转化生长因子Β1 大鼠 

分 类 号：R587.2[医药卫生—内分泌]