强直性脊柱炎患者HLA-B~＊27亚型与KIR基因相关性研究  

Association between the subtypes of HLA-B~＊27 and the polymorphisms of KIR gene

作　　者：阎欢[1] 王薇[1] 胡志东[1] 

机构地区：[1]天津医科大学总医院检验中心,天津300052

出　　处：《陕西医学杂志》2012年第3期281-284,共4页Shaanxi Medical Journal

摘　　要：目的:探讨强直性脊柱炎患者人类白细胞抗原B27(HLA-B*27)各基因亚型与杀伤细胞免疫球蛋白样受体(KIR)基因多态性的关联性。方法:采用序列特异性引物聚合酶链反应(PCR-SSP)法,分析60例强直性脊柱炎(AS)且HLA-B*27阳性患者的HLA-B*27亚型和KIR基因各基因频率,并以30例无血缘关系的正常人做对照,进行相关性分析。结果:HLA-B*27阳性者中以B*2704和B*2705两种亚型为主,两个HLA-B*27亚型的KIR基因频率无统计学差异。AS组中抑制型KIR2DL2、活化型KIR2DS5基因频率较正常对照组显著降低,差异有统计学意义,活化型KIR2DS4基因频率较正常对照组显著升高,差异有统计学意义。结论:强直性脊柱炎患者较正常对照KIR基因有显著差异,KIR基因在强直性脊柱炎发病机理上扮演重要角色,但HLA-B*27亚型与KIR基因多态性无显著关联性。Objective： To investigate whether the subtypes of HLA-B＊27 has relation with the polymorphisms of KIR gene.Methods： Polymerase chain reaction-sequence specific primers（PCR-SSP） method was employed for genotyping the subtypes of HLA-B＊27 and KIR frequency from 60 HLA-B＊27 positive patients and 30 healthy donors.Results： B＊2704 and B＊2705 were the majority HLA-B＊27 subtypes,the frequencies of KIR genes between them had no statistically significant difference.Gene frequencies of KIR2DL2 and KIR2DS5 were significantly lower than the control group.Gene frequency of KIR2DS4 was statistically significant higher in the patient group than in the control group（P=0.001）.Conclusion：The subtypes of HLA-B＊27 is not remarkably associated with the polymorphisms of KIR gene.

关 键 词：@人类白细胞抗原B＊27 @杀伤细胞免疫球蛋白样受体 脊柱炎 强直性/免疫学 聚合酶链反应 

分 类 号：R392[医药卫生—免疫学]