金银花中绿原酸和木犀草苷的薄层色谱鉴别方法研究  

The study of the TLC identification method of Luteoloside and Chlorogenic Acid in Lonicera japonica Thunb.

作　　者：王永慧[1] 孙荣进[2] 叶方[1] 

机构地区：[1]湖北医药学院附属太和医院药学部,湖北十堰442000 [2]湖北医药学院药学院,湖北十堰442000

出　　处：《中国医药导报》2012年第11期136-137,共2页China Medical Herald

摘　　要：目的建立金银花中绿原酸和木犀草苷的薄层色谱鉴别方法。方法参照《中国药典》2010年版一部附录ⅥB试验TLC法,分别考察供试品样品的制备,展开剂的选择,以及显色方法。结果以水为溶剂热回流提取制备试验样品,以乙酸乙酯-丙酮-甲酸-水(7∶3∶1∶1.2)为展开剂,分别喷以5%的三氯化铝乙醇溶液,至紫外光灯(365 nm)下观察鉴定木犀草苷。喷以1%三氯化铁试液与1%铁氰化钾试液(1∶1)混合溶液的上清液,显色鉴定绿原酸。结论所建立的方法简便可行,重现性好,可用于金银花中绿原酸和木犀草苷的鉴定。Objective To establish the TLC identification method of Luteoloside and Chlorogenic acid in Lonicera japonica Thunb..Methods According to TLC testing in Appendix Ⅵ B of Chinese Pharmacopeia（2010 edition,1 volume）,the test sample preparation,the agent of choice and the chromogenic method were researched.Results The TLC conditions were as follows： the developing solvent was mixtured of ethyl acetate-acetone-formic acid-water（7∶3∶1∶1.2） and 5% ethanol solution of aluminum chloride to UV light（365 nm） identification of Luteoloside,1% ferric chloride solution and potassium ferricyanide solution（1∶1） mixture of the supernatant used as color-developing agent identification of chlorogenic acid.Conclusion The method is simple and feasible with good reproducibility and can be used to identification of Luteoloside and Chlorogenic acid in Lonicera japonica Thunb..

关 键 词：金银花 木犀草苷 绿原酸 薄层色谱法 

分 类 号：R284.1[医药卫生—中药学]