178名新生儿单纯疱疹病毒1型和巨细胞病毒血清抗体检测分析  

Detection of antibodies to herpes simplex virus type 1 and human cytomegalovirus in 178 neonates

作　　者：任鹏[1] 姚娟[1] 金艳[2] 汪晓婷[1] 葛俊[1] 李法锦[1] 赵蕾[1] 吉阳[1] 江龙凤[1] 王明丽[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥230032 [2]合肥市妇幼保健院,230001

出　　处：《国际生物制品学杂志》2012年第2期64-66,共3页International Journal of Biologicals

基　　金：国家自然科学基金（30872253）;安徽省科技攻关计划（08010302179）

摘　　要：目的了解新生儿单纯疱疹病毒1型（herpes simplex virus type 1, HSV-1 ）和人巨细胞病毒（ human cytomegalovirus, HCMV）血清抗体阳性情况及其与早产儿临床表现的关系。方法收集新生儿血清共178份（其中健康产妇顺产新生儿脐带血血清114份，早产儿外周血血清64份），分别用HSV-1和HCMVIgG、IgMELISA试剂盒进行检测。采用矿检验对结果进行比较。结果178名新生儿中，血清HSV-1IgG阳性率为83．70％，IgM为阴性；HCMVIgG和IgM阳性率分别为94．38％和0．56％。在114名顺产新生儿中，HSV-1IgG和IgM阳性率分别为78．07％和0．00％，HCMVIgG和IgM阳性率分别为94．74％和0．88％，HSV-1IgG和HCMVIgG共阳性率为73．68％。在64名早产儿中，HSV-1IgG和IgM阳性率分别为93．75％和0．00％，HCMVIgG和IgM阳性率分别为93．75％和0．00％，HSV-1IgG和HCMVIgG共阳性率为87．50％。早产儿与顺产儿的HCMVIgG阳性率差异无统计学意义（χ1=0．07，P〉0．05）；但早产儿HSV-1IgG阳性率以及HSV-1IgG和HCMVIgG共阳性率均高于顺产儿，且差异有统计学意义（χ2值分别为7．38和4．65，P值均〈0．05）。HSV-1IgG和HCMVIgG阳性早产儿出现胎膜早破、胎盘早剥、羊水过少、羊水污染和皮肤异常的几率明显高于阴性早产儿。结论本次检测的新生儿HSV-1IaG和HCMVIzG阳性率较高，且两者阳性的早产儿可能伴随胎膜早破等临床表现。Objective To understand serum positive rates of herpes simplex virus type 1 （ HSV-1 ） and human cytomegalovirus （HCMV） in 178 neonats and their relationship with clinical manifestation of premature infants. Methods A total of 178 neonatal serum samples were collected, including 114 umbilical cord sera from normally delivered newborns and 64 blood sera from premature infants. IgG and IgM antibodies to HSV-1 and HCMV were detected with ELISA. Thex2 test was adopted for the comparisons. Results In 178 neonats, HSV-1 IgG and IgM positive rates were 83.70% and 0.00% , HCMV IgG and IgM positive were 94.38% and 0.56%, respectively. In 114 normally delivered newborns, HSV-1 IgG and IgM positive rates were 78.07% and 0.00%, HCMV IgG and IgM positive were 94. 74% and 0. 88%, respectively. The positive rate of both HSV-1 IgG and HCMV IgG was 73.68%. In 64 premature infants, HSV-1 IgG and IgM positive rates were 93.75% and 0.00% , HCMV IgG and IgM positive rates were 93.75% and 0.00% , respectively. The positive rate of both HSV-1 IgG and HCMV IgG was 87.50%. The HCMV IgG positive rates between premature and normally delivered neonates were not statistically significant （χ2 = 0. 07, P 〉 0.05 ） ; while HSV-1 IgG positive rate and both HSV-1 IgG and HCMV IgG positive rate in premature neonates were significantly higher than those in normally delivered newborns （χ2 = 7.38, P 〈 0. 05 ; χ2 = d. 65, P 〈 0. 05 ）. The premature infants with HSV-1 IgG and HCMV IgG positive had higher risk of premature rupture of membranes, placental abruption, oligohydramnios, amniotic fluid contamination and skin abnormalities. Conclusions The positive rates of HSV-1 IgG and HCMV IgG are high in neonates. The premature infants with HSV-1 IgG and HCMV IgG positive may be associated with premature rupture of membranes and other clinical manifestations,

关 键 词：巨细胞病毒 疱疹病毒1型 人 酶联免疫吸附测定 婴儿  新生 

分 类 号：R722[医药卫生—儿科]