吲哚美辛对COPD大鼠TNF-α与Bcl-2和Bax表达的影响  

The Influence of Indomethacin on the Expression of TNF-α and Bcl-2 and Bax in Chronic Obstructive Pulmonary Disease Rat Model

作　　者：周兰英[1] 孙圣华[2] 高健 宋薇[1] 杨小仙[1] 唐文祥[2] 

机构地区：[1]怀化医学高等专科学校,湖南怀化418000 [2]中南大学湘雅三医院呼吸科,湖南长沙410013 [3]湘西自治州人民医院呼吸内科,湖南吉首416000

出　　处：《现代生物医学进展》2012年第6期1044-1047,共4页Progress in Modern Biomedicine

基　　金：怀化医学高等专科学校资助项目(2010KY07)

摘　　要：目的:探讨吲哚美辛对慢性阻塞性肺疾病(COPD)模型大鼠趾长伸肌TNF-α及Bcl-2和Bax表达的影响。方法:100只健康Wistar大鼠随机分为模型组和对照组(A组)。模型组采用熏香烟和气管内滴注猪胰蛋白酶(PEE)法建立COPD模型大鼠,以低于A组大鼠平均体重的90%判断发生营养不良的标准,将模型组大鼠分为COPD营养正常组(B组)和COPD营养不良组,COPD营养不良组随机化原则分为COPD营养不良生理盐水组(C组)、吲哚美辛1组(D组)、吲哚美辛2组(E组)、吲哚美辛3组(F组)。A、B、C三组灌注等量生理盐水,D、E、F组给予不同剂量吲哚美辛(IND)灌胃干预。TUNEL法测定趾长伸肌细胞凋亡率,免疫组织化学法测定Bcl-2、Bax蛋白表达,双抗体夹心法测定血清及趾长伸肌肿瘤坏死因子α(TNF-α)浓度。结果:吲哚美辛干预后,E组趾长伸肌细胞凋亡率、Bcl-2、Bax的蛋白表达、趾长伸肌匀浆和吲哚美辛干预后血清TNF-α浓度低于C、D、F组,但高于A、B组,P<0.05;E组趾长伸肌Bcl-2蛋白表达高于C、D、F组,低于A、B组,P<0.05。结论:Bcl-2、Bax及TNF-α参与COPD模型大鼠趾长伸肌细胞凋亡,适当剂量的吲哚美辛可改善COPD营养不良模型骨大鼠趾长伸肌的凋亡。Objective： To investigate the effect of indomethacin on the expression of Bcl-2,TNFα and Bax in extensor digitorum longus in the COPD rat＇s models.Methods： 100 healthy Wistar rats were randomly divided into model group and control group（group A）.The model group rat was performed with intratracheal instillation of porcine pancreatic elastase（PEE） and exposed to cigarette smoke to establish COPD models.Malnutrition was defined if the body weight of the rats in the model group was lower than 90% of the mean body weight of the control group.All model rats were divided into non-malnutrition COPD group（group B） and malnutrition COPD group,and the malnutrition COPD group rats were randomly divided into four groups： physiologic saline control group（group C）,Indomethacin intervention group 1（group D）,Indomethacin intervention group 2（group E） and Indomethacin intervention group 3（group F）.Isotonic physiologic saline was administered to the group A,B and C,different dose of Indomethacin was administered to group D,E,F by gavage.The apoptosis rates of the cell in extensor digitorum longus muscle were measured by TUNEL method,the Bcl-2,Bax protein expression and the concentrations of TNFα in the serum,the extensor digitorum longus muscle from these rats were measured by immunohistochemical method,take the method of double mono-cloned antibody for detecting the concentrations of TNF-alpha in the serum and extensor digitorum longus muscle homogenates from these rats.Results： After indomethacin intervention,the rates of muscle cell apoptosis,the concentration of TNFα,Bcl-2 and the Bax protein expression,extensor digitorum longus muscle homogenates,the concentrations of TNF-alpha in the serum in the Indomethacin intervention group 2（group E） were lower than those in the group C,D,F and higher than those in the group A,B（P0.05）,the Bcl-2 protein expression in extensor digitorum longus muscle in group E was higher than that in C,D,F group and was lower than that in group A,B（P0.05

关 键 词：慢性阻塞性肺疾病 骨骼肌 细胞凋亡 BCL-2 BAX TNF-Α 

分 类 号：Q95-3[生物学—动物学] Q291