柴胡渗湿汤对哮喘大鼠血清中IL-5及IL-13影响的实验研究(英文)  

Study on the Effects of Radix Bupleuri Dampness-Percolating Decoction on Serum IL-5 & IL-13 in Rat Asthma Model

作　　者：闫云霞[1] 王燕青 周兆山 郭桂明[1] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]青岛市海慈医疗集团,山东青岛266033

出　　处：《现代生物医学进展》2012年第7期1251-1256,共6页Progress in Modern Biomedicine

摘　　要：目的:观察柴胡渗湿汤对哮喘大鼠血清中IL-5及IL-13含量的影响,探讨柴胡渗湿汤治疗哮喘的作用机制。方法:将84只雄性Wi star大鼠按随机数字表法分为正常对照组、模型对照组、地塞米松组、定喘汤组、柴胡渗湿汤低剂量组、柴胡渗湿汤中剂量组、柴胡渗湿汤高剂量组,每组12只,采用卵蛋白制作大鼠哮喘模型,予相应药物干预。用酶联免疫吸附试验(ELISA)法检测各组大鼠血清中IL-5及IL-13水平。实验数据采用SPSS11.5统计软件进行分析。结果:实验后各组死亡率比较均无差异,P>0.05;模型对照组与正常对照组血清中IL-5及IL-13含量水平有显著差异,表明大鼠哮喘模型存在着血清IL-5及IL-13含量异常增高的病理状态;与模型对照组相比较,各治疗组血清中IL-5及IL-13的含量明显降低,其中尤以地塞米松组、柴胡渗湿汤中剂量组和柴胡渗湿汤高剂量组的作用更明显。结论:柴胡渗湿汤治疗哮喘的作用机制与降低IL-5及IL-13的水平有关;上述作用具有一定的量效关系,但并不因给药剂量的增加而增加。Objective： To investigate the effects of radix bupleuri dampness-percolating decoction on the IL-5 and IL-13 in the rat asthma model serum and its function mechanism of asthma treatment.Methods： Eighty-four healthy male Wistar rats were divided by random number method into normal control group,model control group,dexamethasone group,dingchuan decoction group,low dosage radix bupleuri dampness-percolating decoction group,medium dosage radix bupleuri dampness-percolating decoction group and high dosage radix bupleuri dampness-percolating decoction group,with 12 in each group.Then the model of rat asthma was made with ovalbumin and intervened by relevant medicine.The IL-5 and IL-13 in rat serum from each group were tested by enzyme-linked immunosorbent assay（ELISA）;experimental data were analyzed by SPSS11.5 statistical software.Results： Mortality ratio of each group after experiment had no difference,P0.05.There existed significant difference between the model control group and the normal control group in terms of serum IL-5 and IL-13 content,which indicated a pathological state that serum IL-5 and IL-13 in rat asthma model abnormally increased.Compared with model control group,IL-5 and IL-13 in treatment groups＇ serum obviously decreased,especially to the dexamethasone group,medium dosage radix bupleuri dampness-percolating decoction group and high dosage radix bupleuri dampness-percolating decoction group.Conclusion： The function mechanism of radix bupleuri dampness-percolating decoction for asthma treatment is related to reduction of the IL-5 and IL-13 level.A certain dose-response relationship existed in the said effect,but the effect does not become stronger with higher dosage.

关 键 词：哮喘 柴胡渗湿汤 大鼠 IL-5 IL-13 

分 类 号：Q95-3[生物学—动物学] R562.25[医药卫生—呼吸系统]