RP-HPLC同时测定野菊花中绿原酸、木犀草苷和蒙花苷的含量  

RP-HPLC Simultaneous Determination of Chlorogenic Acid,luteolin-7-O-β-D-glucoside and Linarin in Chrysanthemi Indici Flos

作　　者：郭美兰[1] 敬应春[1] 蔡国琴[1] 

机构地区：[1]上海市中药研究所,上海201401

出　　处：《中国现代中药》2012年第4期10-13,共4页Modern Chinese Medicine

基　　金：上海市科学技术委员会中药现代化专项(09DZ1977300)

摘　　要：目的:建立同时测定野菊花中绿原酸、木犀草苷和蒙花苷3个主要活性成分含量的方法。方法:采用RP-HPLC法,选用Kromasil-C18(250mm×4.6mm,5μm,)色谱柱;以乙腈-0.05%磷酸溶液为流动相(梯度洗脱);流速为1.0mL.min-1;检测波长为334nm;柱温为30℃。结果:绿原酸、木犀苷和蒙花苷分别在40.44～647.04ng(r=0.9998)、19.50～311.92ng(r=0.9997)和253.62～4057.82ng(r=0.9999)范围内线性关系良好;平均回收率分别为100.96%、99.99%和98.94%,RSD均<2.0%。22批野菊花药材中绿原酸含量为0.108%～0.545%,木犀苷含量为0.029%～0.393%,蒙花苷含量为0.028%～3.518%。结论:本方法简便准确、稳定可靠,可以用于野菊花药材的含量测定。Objective： To establish a method for the determination of three active ingredients, chlorogenic acid,luteolin-7-O-β-D-glucoside,and Linarin in Flos Chrysanthemi Indici. Methods： A Kromasil -Cls （250 mm ×4.6mm,5μmm）column was used with the mobile phase （gradient elution） being acetonitrile-O. 05% phosphate acid , flow rate being 1.0 mLomin-1, detecting wavelength being 334 nm, the cloum temperature being 30℃. Results： The linear response range from 40. 44 - 647.04 ng（ r = 0. 999 8, n = 5 ） of chlorogenic acid, 19.50 - 311.92 ng（ r = 0. 999 7, n = 5 ） of luteolin-7-O-β-D-glucoside, 253.62- 4 057.82 ng （ r = 0. 999 9, n = 5 ） of Linarin. The average recoveries （ n = 6 ） chlorogenic acid, luteolin-7-O-β-D-glucoside, and Linarin were 100. 96%, 99.99%, and 98.94 % respectively. The average recoveries of chlorogenic acid, luteolin-7-O-β-D-glucoside, and Linarin were 100. 96%, 99. 99% ,and 98.94 % with RSD less than 2. 0%. Conclusion： The method is simple , accurate, reproducible and stable , and can, be used for the quality control of Flos Chrysanthemi Indici.

关 键 词：野菊花 RP—HPLC法 绿原酸 木犀草苷 蒙花苷 

分 类 号：R286.0[医药卫生—中药学]