特异性microRNA-135a在变应性鼻炎小鼠鼻黏膜中的表达  

Expression of Specific MicroRNA-135a in Nasal Mucosa of Allergic Rhinitis Mice

作　　者：罗燕云[1,2] 邓玉琴[1] 陶泽璋[1] 

机构地区：[1]武汉大学人民医院耳鼻咽喉-头颈外科,湖北武汉430070 [2]广州军区武汉总医院耳鼻喉科

出　　处：《华南国防医学杂志》2012年第2期102-105,共4页Military Medical Journal of South China

基　　金：国家自然科学基金项目(81070766)

摘　　要：目的研究特异性microRNA-135a(miR-135a)在变应性鼻炎(allergic rhinitis,AR)小鼠鼻黏膜中的表达,探讨其对AR的免疫调控作用。方法采用卵清蛋白(ovalbumin,OVA)腹腔注射及滴鼻建立AR的小鼠模型,以叠加法进行行为学评分,酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测血清中特异性OVA-sIgE浓度,SYBR Green实时荧光定量逆转录聚合酶链反应(real-time quantitative reverse transcription polymerase chain reaction,qRT-PCR)检测AR小鼠鼻黏膜中转录因子(T-box expressed in T cells,T-bet;GATA binding protein 3,GATA-3)和细胞因子(interferon gamma,IFN-γ;interleukin 4,IL-4)的mRNA表达,以生理盐水组小鼠为对照,确认造模成功。应用Bulge-LoopTMqRT-PCR检测小鼠鼻黏膜中特异性miR-135a表达。结果叠加法记录AR组小鼠挠鼻(鼻痒)、喷嚏、清涕的行为学评分,总分均超过5分,对照组小鼠均低于5分;AR组小鼠的血清中OVA-sIgE浓度高于对照组,鼻黏膜中T-bet和IFN-γ的mRNA表达低于对照组,而GATA-3和IL-4的mRNA表达高于对照组,其差异均有统计学意义。AR组小鼠鼻黏膜中特异性miR-135a表达低于对照组,差异有统计学意义。结论 AR小鼠鼻黏膜中特异性miR-135a低表达可能通过其靶点GATA-3的mRNA高表达在辅助性T淋巴细胞(T helper cell,Th)亚群Th2细胞的极性分化中发挥重要作用,对导致AR的Th1/Th2失衡机制产生重要的免疫调控作用。Objective To study the expression of specific microRNA-135a（miR-135a） in nasal mucosa of allergic rhinitis（AR） mice and research the immune regulation of miR-135a on AR. Methods AR mouse model was established by ovalbumin（OVA） peritoneal injection and nasal drop,and the control group by normal saline.The mice were ethologically evaluated by addition method.Specific serum OVA-sIgE concentration was detected by enzyme-linked immunosorbent assay（ELISA） method.Relative mRNA expressions of transcription factor（T-box expressed in T cells,T-bet and GATA binding protein 3,GATA-3） and cytokines including interferon-gamma（IFN-γ） and interleukin 4（IL-4） were detected by SYBR Green real-time quantitative polymerase chain reaction（qPT-PCR） to assure the success of AR model building.Expression of specific miR-135a in the nasal mucosa of the mice was detected with Bulge-LoopTM qRT-PCR method. Results The ethologically scores of the AR mice were all over 5,and of control group all below 5.Serum OVA-sIgE concentration of AR group was significantly higher,relative mRNA expressions of T-bet and IFN-γ in nasal mucosa of AR group were significantly lower,and expressions of GATA-3 and IL-4 were significantly higher than those of the control group.Expression of specific miR-135a in the nasal mucosa of AR mice was significantly lower than that of control group. Conclusion The low expression of specific miR-135a in the nasal mucosa of AR mice may play an important role in the differentiation of Th2 sub-group through the high mRNA expression of its target GATA-3,and thus regulates the imbalance of Th1/Th2 which results in AR.

关 键 词：MICRORNA 变应性鼻炎 GATA-3 辅助性T淋巴细胞 

分 类 号：R765.213[医药卫生—耳鼻咽喉科]