大鼠多发性大动脉炎模型的建立  

Establishment of Takayasu Arteritis Model in Rat

作　　者：洪志鹏[1] 陈福真[1] 符伟国[1] 徐欣[1] 孙宝荣[2] 纪元[2] 钟慈声[3] 

机构地区：[1]上海医科大学中山医院血管外科,上海200032 [2]上海医科大学中山医院病理科,上海200032 [3]上海医科大学基础医学院电镜室,上海200032

出　　处：《上海医科大学学报》2000年第1期20-23,共4页Journal of Fudan University(Medical Science)

摘　　要：目的　建立大鼠多发性大动脉炎模型，并探讨其发病机制。方法　１０周龄雌性ＳＤ大鼠４０只，随要分为４组：１个对照组（ＮＳ）和３个实验组。对照组每天给予生理盐水２ｍｌ灌胃，实验组分别给予己烯雌酚１ｍｇ／（ｋｇ·ｄ）（ＤＥＳ１组）、２ｍｇ／（ｋｇ·ｄ）（ＤＥＳ２组）和２ｍｇ／（ｋｇ·ｄ）加三苯氧胺１ｍｇ／（ｋｇ·ｄ）（ＴＡＭ组）胃饲３个月后，观察病理和超微结构改变。结果　病变动脉呈灰白色，管壁僵硬，与无明显病变动脉交替出现；光镜下平滑肌细胞聚集成团束状，晚期被大量纤维组织所取代；电镜下变性平滑肌细胞核膜模糊或消失，内质网扩张，线粒体肿胀，在增生的平滑肌细胞周围有许多胶原纤维，病变晚期大量胶原纤维中夹杂萎缩的平滑肌细胞。ＤＥＳ１组、ＴＡＭ组和对照组未发现明显异常。结论　本实验以雌激素诱发大鼠产生类似于人类多发性大动脉炎的疾病模型，提示雌激素参与本病的发生发展过程。Purpose To investigate the relationship between estrogen and pathogenesis of Takayasu arteritis. Methods Forty female 10 week old SD rats were randomly divided into 4 groups,one control group(group NS) and three treatment groups(Group DES1,DES2 and TAM).In control group,rats were given 2 ml/d normal saline(NS).In treatment groups rats received 1 mg/(kg·d) diethylstilbestrol (DES),2 mg/(kg·d) DES,and 2 mg/(kg·d) DES plus with 1 mg/(kg·d) tamoxifen(TAM)respectively for 3 months.The whole aorta and its major branches were dissected for pathologic and ultrastructral observations. Results In group DES2,the arteries with pathological changes were showed grey colour with the stiff wall,and seperated by normal parts.Under the light microscope,the proliferous smooth muscle cells(SMC)were gathered for mass or bundle.In the late stage,SMC were replaced by fibrous tissues.Under the electron micros cope,dilation of endoplasmic reticula,swollen of mitochondia and disappearnce or obscurity of nucleus membrane were observed in degeneration of SMC.There were many collagen fibers around the proliferous or atrophy SMC.In group NS,DES1 and TAM,vascular in morphology was normal. Results In group DES2,the arteries with pathological changes were showed grey colour with the stiff wall,and seperated by normal parts.Under the light microscope,the proliferous smooth muscle cells(SMC)were gathered for mass or bundle.In the late stage,SMC were replaced by fibrous tissues.Under the electron micros cope,dilation of endoplasmic reticula,swollen of mitochondia and disappearnce or obscurity of nucleus membrane were observed in degeneration of SMC.There were many collagen fibers around the proliferous or atrophy SMC.In group NS,DES1 and TAM,vascular in morphology was normal. Conclusions This study used estrogen to induce rat TA model was what is simlilar to human counterpart The results indicate that estrogen play an important role in initial and progression of TA.

关 键 词：多发性 大动脉炎 雌激素 雌激素受体 大鼠 

分 类 号：R654.3[医药卫生—外科学]