锰染毒大鼠的脑组织神经生化分析  

Neurochemical Analysis of Brain Tissue of Rats Exposed to Manganese

作　　者：林洁[1] 陈自强[1] 梁友信[1] 葛琳娜[1] 

机构地区：[1]上海医科大学公共卫生学学院劳动卫生学教研室,上海200032

出　　处：《上海医科大学学报》2000年第1期32-34,共3页Journal of Fudan University(Medical Science)

摘　　要：目的　研究锰对单胺类递质的影响及可能的细胞保护机制。方法　大鼠经饮水染锰（ＭｎＣｌ２·４Ｈ２Ｏ２．５和０．５ｇ／Ｌ）９０ｄ后，取脑组织测定纹状体多巴胺（ＤＡ）、高香草酸（ＨＶＡ）、３，４双羟苯乙酸（ＤＯＰＡＣ），５羟色胺（５ＨＴ）、５羟吲哚乙酸（５ＨＩＡＡ）含量，以及脑组织丙二醛（ＭＤＡ）、谷胱甘肽含量和超氧化物歧化酶（ＳＯＤ）活性。结果　高剂量组的５ＨＩＡＡ和ＨＶＡ含量明显低于对照组和低剂量组，ＳＯＤ活性随染毒剂量增加而增高，ＤＡ、ＤＯＰＡ含量与谷胱甘肽含量呈负相关，５ＨＩＡＡ、ＨＶＡ含量ＳＯＤ活性呈负相关。结论　锰可影响ＤＡ和５ＨＴ的代谢，而巯基和ＳＯＤ有一定的保护作用。Purpose To study the effect of manganese on monoamines and possible cellular defense mec hanism . Methods Male SD rats were given drinking water containing manganese chloride(MnCl\-2·4H\-2O 0.5 and 2.5 g/L respectively) for 90 days.At the end of experiment,all rats of the exposed groups and control group were decapitated,there brains were removed and analyzed levels of dopamine(DA),3,4 dihydroxyphenylacetic acid(DOPAC),homovanillic acid(HVA),5 hydroxytryptamine(5 HT) and 5 hydroxyindole acetic acid(5 HIAA) in striatum;lipid peroxide,glutathione and superoxide dismutase(SOD) activity in brain. Results 5 HIAA and HVA levels in striatum decreased significantly in heavy exposed group;SOD activities increased in exposed groups.Correlation analysis indicated that DA,DOPA levels were negatively correlated with glutathione,while 5 HIAA,HVA levels was negatively correlated with SOD activity. Results 5 HIAA and HVA levels in striatum decreased significantly in heavy exposed group;SOD activities increased in exposed groups.Correlation analysis indicated that DA,DOPA levels were negatively correlated with glutathione,while 5 HIAA,HVA levels was negatively correlated with SOD activity. Conclusions Manganese may effect the metabolism of DA and 5 HT,and glutathione and SOD may partly block this course.

关 键 词：单胺类递质 细胞保护机制 锰中毒 大鼠 脑组织 

分 类 号：R595.206[医药卫生—内科学] R741.02[医药卫生—临床医学]