急性肺损伤大鼠颗粒溶素与白介素-2相关性表达的研究  

The Correlative Study on the Expressions of Granulysin and Interleukin-2 in Acute Lung Injury Rats

作　　者：盖菁菁[1] 王娟[1] 王金平[1] 

机构地区：[1]潍坊医学院人体解剖学教研室,山东潍坊261053

出　　处：《潍坊医学院学报》2012年第2期132-134,F0003,共4页Acta Academiae Medicinae Weifang

摘　　要：目的 在大鼠急性肺损伤模型中,观察肺组织颗粒溶素表达及血清白介素-2水平的改变及相关性,探讨急性肺损伤的机制.方法 36只Wistar大鼠随机分成实验组（LPS组）和正常对照组（NS组）,LPS组腹腔注射脂多糖（LPS）制备急性肺损伤（ALI）模型,NS组注射等量生理盐水,并于注射后6,18,30h取材.通过ELISA法检测血清白介素-2变化,并取肺组织行HE染色观察病理改变,免疫组化法测定肺组织颗粒溶素的表达.结果 LPS组大鼠病理改变表现为肺组织受损,水肿、出血、渗出明显,符合急性肺损伤标准;LPS组各时点血清白介素-2水平和肺组织GNLY表达均较对照组有不同程度增加（P〈0.05）.结论 急性肺损伤大鼠血清白介素-2水平与颗粒溶素表达有相关性,共同参与急性肺损伤的致病和免疫防御过程.Objective To oberve the correlative expression of granulysin （GNLY） and interleukin-2 （IL-2） in the acute lung in- jury（ ALI） rats stimulated with lipopolysaccharide（LPS） at different time point,and to explore the pathogenic mechanism of ALI. Methods Thirty-six healthy adult wistar rats were randomly divided into 2 groups （ n = 18 ） ： normal control groups （ NS group）} and experimental group （ LPS group）. Rats in the LPS group were injected with LPS（4mg/kg） intraperitoneally in order to induce acute lung injuryC ALI） ,while the NS group rats were injected with the same amount of normal saline. Six rats in each group were killed at 6,18 and 30 hours after LPS or NS injection. Then their lungs were harvested for HE staining,and the expressions of GNLY in lung tissue were assayed by immunohistochemis- try. Results In LIPS group, histopathologically, lung tissues were damaged severely and there were lots of inflammation cells infiltration, edema, and hemorrhage in pulmonary interstitial spaces which suggested ALI. Compared with normal control group,the expressions of GNLY and IL-2 in blood serum in LPS group increased significantly（ P 〈0.05 ） ,and there was correlation between the GNLY and IL-2 expression. Conclusion In ALl rats,the expression levels of GNLY are correlated with that of IL-2 in blood serum. It is close relation among the expression of GNLY and the ALI occurrence and progress. High levels of GNLY would be activated and up-regulated by a series of inflammatory cytokines such as IL-2,etc.

关 键 词：肺损伤 急性 脂多糖 颗粒溶素 白介素-2 

分 类 号：R446.61[医药卫生—诊断学]