麻风流行区饮用水中麻风杆菌的分子生物学检测  

Molecular detection of M.leprae from environmetal water in leprosy endemic area

作　　者：邢燕[1] 刘健[1] 温艳[1] 翁小满[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京热带医学研究所,100050

出　　处：《中国麻风皮肤病杂志》2012年第4期238-241,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金项目(No.30670111)

摘　　要：目的:检测麻风病流行区饮用水中麻风杆菌DNA,以探讨其对麻风流行区麻风持续流行的影响。方法:分别从云南丘北县有、无患者村和北京非流行区乡村采集70份水样,提取DNA、PCR扩增后测序检测样品中麻风杆菌DNA。结果:流行区36.7%(22/60)的水样含麻风杆菌DNA,而非流行区水样中未检出麻风杆菌。流行区有患者的村庄中,患者和健康者家庭内饮水中麻风杆菌的检出率有统计学差异。结论:麻风流行区患者家庭饮用水中麻风菌的存在可能与持续性流行有关,但须进一步研究。Objective： To detect M. leprae DNA from drinking water in order to explore the impact on the transmission of leprosy in endemic area. Me,otis： Seventy water samples were collected from ＂patient＂ villages and ＂ non- patient＂ villages in Qiubei county, Yunnan province and from the villages in non- endemic area of Beijing. M. leprae DNA extraction was performed and sequenced with PCR amplification. Results： Twenty - two （36.7 % ） out of 60 water samples from endemic area were identified as having M. leprae DNA, while M. leprae DNA was not detected from water samples in non - endemic area. In endemic area, there was a significant difference in the detection rate of M. leprae in domestic drinking water between patients and healthy persons. Conclusion： The M. leprae contained in environmental water may be related to the continuous transmission of leprosy in endemic area, which needs to be studied in future.

关 键 词：麻风病 麻风杆菌 环境水 

分 类 号：R755[医药卫生—皮肤病学与性病学]