毛果芸香碱刺激与非刺激条件下家兔体液中氯胺酮含量分析  

STUDY ON THE CONTENT OF KETAMINE IN RABBITS BODY FLUID UNDER THE CONDITION OF PILOCARPINE STIMULUS AND NON STIMULUS

作　　者：钱玮[1] 李鹏旺[1] 刘俊芳[1] 王玉瑾[1] 

机构地区：[1]山西医科大学法医学院,太原030001

出　　处：《中国药物依赖性杂志》2012年第2期118-121,共4页Chinese Journal of Drug Dependence

基　　金：山西省自然科学基金资助项目(2007011105);"十一五"国家科技支撑计划项目(2007BAK26B05);山西省科技攻关项目(20110321082);山西医科大学博士启动基金(03200814)

摘　　要：目的:比较研究毛果芸香碱刺激和非刺激条件下家兔唾液、血液和尿液中氯胺酮及代谢物去甲氯胺酮的含量。方法:实验家兔分为毛果芸香碱刺激组和非刺激组,分别于氯胺酮染毒前和染毒后不同时间点收集唾液、血液和尿液。气相色谱/质谱联用(GC/MS)全扫描定性、气相色谱(GC)定量分析血液、唾液和尿液样品中氯胺酮及去甲氯胺酮的浓度。采用两因素重复测量方差分析方法分别比较刺激组和非刺激组之间氯胺酮及去甲氯胺酮在唾液、血液和尿液中平均检出量有无统计学意义。结果:毛果芸香碱刺激组和非刺激组之间氯胺酮及去甲氯胺酮在唾液、血液和尿液中平均检出量均无显著性差异(P>0.05)。结论:唾液中氯胺酮的采集可用毛果芸香碱刺激或非刺激法,但刺激法可以增加唾液分泌,易采集且增加采集次数和采集量。Objective： Compared the content of ketamine and norketamine in rabbits body fluid between the pilocarpine stimulus group and non stimulus group. Methods：The acute toxic rabbits were divided into two groups, one group was stimulated by pilocarpine and another wasn＇ t stimulated. Collected their saliva, plasma and urine at different time points after administration. The contents of ketamine and norketmnine were qualitatively analyzed by GC/MS and quantitatively analyzed by GC. Compared the contents of ketamine and norketamine between two groups by repeated measure variance analysis. Results：There was no significant difference between pilocarpine stimulus group and non stimulus group（P 〉 0. 05 ）. Conclusion：The methods of pilocarpine stimulus and non stimulus can be applied to collecting saliva for detecting ketamine. Pilocarpine stimulus method can increase salivary secretion, collection frequency and collection quantity.

关 键 词：氯胺酮 毛果芸香碱 唾液 血液 尿液 

分 类 号：R917[医药卫生—药物分析学]