父源性HLA-B基因与子痫前期相关性的研究  

Study on the correlation between paternal HLA-B gene and preeclampsia

作　　者：张展[1] 刘文霞[2] 张琳琳[1] 贾莉婷[1] 魏伟[2] 

机构地区：[1]郑州大学第三附属医院检验科,河南郑州450052 [2]郑州大学第三附属医院妇产科

出　　处：《中国妇幼保健》2012年第12期1851-1855,共5页Maternal and Child Health Care of China

基　　金：国家自然科学基金〔30872316〕;河南省科技厅项目〔072102310009〕;河南省卫生厅科技攻关项目〔201002011〕

摘　　要：目的:探讨父源性人类白细胞分化抗原HLA-B基因与子痫前期的相关性。方法:利用流式反向特异序列寡核苷酸探针(PCR-SSO)基因分型技术,对中国地区汉族人群中37对子痫前期患者夫妇及38对正常孕妇夫妇的外周血DNA,进行HLA-B等位基因分型;分别比较两组母亲之间和两组配偶之间等位基因及基因型的频率分布,通过妇/夫基因型配伍,比较两组间基因型配伍频率分布的差异。结果:①子痫前期患者和正常晚孕者中检出34个HLA-B等位基因;配偶中检出28个HLA-B等位基因;两组孕妇及其配偶间HLA-B基因频率均无统计学差异;②子痫前期组妇携带HLA-B13夫不携带HLA-B13基因的频率显著高于正常晚孕组(P<0.05);③子痫前期组夫妇均不携带HLA-B13基因的频率显著低于正常晚孕组(P<0.05)。结论:夫妇间的某些特定HLA-B基因配伍模式可能与子痫前期的发病相关。Objective：To explore the correlation between paternal human leucocyte antigen-B（HLA-B） and preeclampsia. Methods：PCR-SSO genotyping technique was used for genotyping of HLA-B alleles in peripheral blood DNA of 37 couples of preeclampsia and 38 couples of normal pregnancy of Han nationality in China;the frequences and distributions of alleles and genotypes among the mothers and their husbands were compared between the two groups,the differences of frequences and distributions of genotypes were compared between the two groups according to genotype compatibility of the mothers and their husbands. Results：Among the patients with preeclampsia and the normal late pregnant women,34 HLA-B alleles were detected;among their husbands,28 HLA-B alleles were detected;there was no significant difference in the frequency of HLA-B gene among the mothers and their husbands between the two groups;the frequencies of women with HLA-B gene and their husbands without HLA-B gene in preeclampsia group were significantly higher than those in normal late pregnancy group（P0.05）.The frequencies of women and their husbands without HLA-B gene in preeclampsia group were significantly lower than those in normal late pregnancy group（P0.05）. Conclusion：Some particular matching modes of HLA-B gene between the couples may be related to the occurrence of preeclampsia.

关 键 词：人类白细胞抗原 子痫前期 HLA-B 多态性 

分 类 号：R714.245[医药卫生—妇产科学]