LPSp对HBV宫内感染脐血单个核细胞分泌IL-4、IFN-γ及TLR4表达的影响  

Effect of LPSp on the secretions of IL-4 and IFN-γ by mononuclear cells and TLR4 expression in umbilical cord blood of pregnant women with HBV intrauterine infection

作　　者：李常虹[1] 陈悦[1] 黄星[1] 王启辉[2] 

机构地区：[1]广西医科大学第一附属医院产科,广西南宁530021 [2]广西医科大学基础医学院微生物学与免疫学教研室

出　　处：《中国妇幼保健》2012年第12期1856-1858,共3页Maternal and Child Health Care of China

基　　金：广西科技厅自然科学基金项目〔桂科攻1140003A-3〕

摘　　要：目的:观察不同浓度LPSp对HBV宫内感染孕妇的脐血单个核细胞分泌IL-4、IFN-γ及细胞表面TLR4表达的影响。方法:选择外周血HBsAg、HBeAg、HBcAb阳性且脐血HBV-DNA阳性孕妇25例为试验组,根据加入LPSp的不同分为:无LPSp组、LPSp 1μg/L组、LPSp 10μg/L组、LPSp 100μg/L组;以外周血HBV标志物为阴性的正常分娩孕妇20例为对照组。分离CBMC并培养,采用ELISA法检测培养上清液中的IFN-γ、IL-4的浓度,用流式细胞仪检测CBMC表面TLR4表达。结果:①LPSp各组中IFN-γ、IL-4水平与无LPSp组比较差异均有统计学意义(P<0.01),IFN-γ水平与LPSp浓度呈正相关(r=0.778),IL-4水平与LPSp浓度呈负相关(r=-0.927)。②TLR4表达与IFN-γ水平呈正相关(P<0.05),TLR4与IL-4呈负相关(P<0.05);TLR4表达水平与LPSp浓度呈正相关(r=0.754)。结论:不同浓度的LPSp可能通过影响TLR4信号传导通路诱导CBMC分泌不同细胞因子,选择性调控Th1/Th2型应答。Objective：To observe the effects of different concentrations of pantoea agglomerans lipopolysaccharid（LPSp） on the secretions of IL-4 and IFN-γ by mononuclear cells and TLR4 expression on the surface of mononuclear cells in umbilical cord blood of pregnant women with HBV intrauterine infection. Methods：Twenty-five pregnant women with positive HBsAg,HBeAg,and HBcAb in peripheral blood and positive HBV-DNA in umbilical cord blood were selected as study group,then they were divided into non-LPSp group,1 μg/L LPSp group,10 μg/L LPSp group,and 100 μg/L LPSp group according to the concentration of LPSp;twenty normal pregnant women with negative HBV markers in peripheral blood were selected as control group.CBMC were isolated and cultured,ELISA was used to detect the concentrations of IFN-γ and IL-4 in liquid supernatant,the expression of TLR4 on the surface of CBMC was detected by flow cytometry. Results：There was significant difference in the levels of IFN-γ and IL-4 between non-LPSp group and the other three groups（P0.01）,there was a positive correlation between IFN-γ level and LPSp concentration（γ=0.778）,while there was a negative correlation between IL-4 level and LPSp concentration（γ=-0.927）.There was a positive correlation between TLR4 expression and IFN-γ level（P0.05）.There was a negative correlation between TLR4 expression and IL-4 level（P0.05）.There was a positive correlation between TLR4 expression and LPSp concentration（γ=0.754）. Conclusion：Different concentrations of LPSp can induce CBMC to secret different cytokines by affecting TLR4 signaling transduction pathway,which can regulate Th1/Th2 response.

关 键 词：LPSP 乙型肝炎病毒 宫内感染 IFN-γ IL-4 TLR4 

分 类 号：R711.74[医药卫生—妇产科学]