草莓叶片和叶柄再生体系的建立  

Plant regeneration from strawberry leaf disks and petioles

作　　者：宋国庆[1] 肖兴国[2] 李绍华[1] 杨晓东 

机构地区：[1]中国农业大学果树系 [2]中国农业大学植物生理生化实验室,北京100094

出　　处：《落叶果树》2000年第2期5-8,共4页Deciduous Fruits

基　　金：国家自然科学基金!项目号 :396 70 519

摘　　要：以红丰和宝交早生品种的组培苗为试材,初步建立了叶圆盘和叶柄片段植株再生体系。红丰的芽苗再生能力高于宝交早生,叶圆盘的再生频率高于叶柄片段,叶圆盘下表面(背面)接触培养基比上表面(正面)更有利于再生。AgNO3有利于芽苗再生,在接种叶圆盘前加入主要刺激芽苗的直接再生,而在愈伤组织形成后加入则主要提高再生频率。再生芽苗在三角瓶中容易生根,移苗入土后生长正常。Plants of strawberry(Fragaria ananassaDuch.) from leaf disks and petiole segments were regenerated with two cultivars Denitoyo and Hokowase.Shoots of Denitoyo were more active than those of Hokowase in regeneration ability, and the leaf disks showed much higher regeneration frequency than petiole segments,especially when their lower surfaces were kept contacted with culture medium. Addition of AgNO 3 to the medium at leaf disk inoculation stage directly stimulated shoot regeneration, while markedly increased shoot regeneration frequency at the time after callus formation. All regenerated shoots rooted easily in vitro and the resulted plants grew normally after transplanting in pots.

关 键 词：草莓 叶圆盘 叶柄 植株再生 AGNO3 

分 类 号：S668.403.5[农业科学—果树学]