骨形成蛋白Ⅰ型受体A在雄激素源性脱发中的表达意义  

Expression of BMP typeⅠA receptor in the scalp of AGA patients and its significance

作　　者：李晓欣[1] 万苗坚[1] 徐峰[1] 冯智英[1] 胡婷[1] 赖维[1] 谢小元[1] 计斌[1] 

机构地区：[1]中山大学附属第三医院皮肤科,广东广州510630

出　　处：《临床皮肤科杂志》2012年第5期267-271,共5页Journal of Clinical Dermatology

基　　金：广东省自然科学基金(10151008901000204)资助项目

摘　　要：目的:探讨骨形成蛋白Ⅰ型受体(BMPR-Ⅰ)A在正常人及雄激素源性脱发(androgenetic alopecia,AGA)患者头皮毛囊中的表达及其意义。方法:采用Westetn blot、免疫组化及RT-PCR分析8例AGA患者额顶部脱发区与枕部非脱发区毛囊及11例正常人头皮毛囊中BMPR-ⅠA蛋白及基因水平表达差异;毛囊分组培养,观察双氢睾酮(dihydrotestosterone,DHT)10^(-9)mol/L对BMPR-ⅠA表达及毛囊生长的影响。结果:各组头皮毛囊均可见BMPR-ⅠA阳性染色。与正常毛囊相比,AGA患者脱发区毛囊阳性染色A值降低(P<0.05)。Western blot结果显示,AGA患者头顶部毛囊BMPR-ⅠA含量较正常人下调,目的蛋白与内参灰度比由1.733±0.058下调为0.396±0.036(P<0.05);AGA枕部毛囊目的蛋白与内参灰度比值为1.574±0.039,与正常人组差异无统计学意义。毛囊培养DHT组与生理盐水对照组相比,目的蛋白与内参灰度比由3.441±0.172下降为0.367±0.026(P<0.05)。RT-PCR结果示,AGA脱发区毛囊BMPR-ⅠAmRNA下调为正常组的53%(P<0.05),AGA枕部无明显下调;毛囊培养DHT组下调为对照组的13%(P<0.05)。结论:AGA脱发区毛囊与DHT组毛囊BMPR-ⅠA表达均降低,提示BMPR-ⅠA表达下降部分是由DHT升高引起;DHT可引起的毛囊生长减慢及退行性变;BMPR-ⅠA表达下降可能是DHT升高的直接作用或者是AGA生长期与退行期毛囊比例下降的代偿机制;其参与AGA发病可能与毛干及内根鞘形成障碍引起毛发脱落有关。Objective： To study the expression of bone morphogenetic protein receptor I A （BMPR- I A） in the scalp of an- drogenetic alopecia （AGA） patients and its significance. Methods： Specimens were obtained from the frontoparietal alopecic scalp and occipital non-alopeeic scalp of 8 AGA patients and healthy scalp of 11 healthy volunteers with informed consent and subjected to Western blot, immunohistochemistry and RT-PCR for the detection of BMPR- I A. Hair follicles were cultured with DHT（dihydrotestosterone）10-gmol/L to observe the growth velocity, morphology of the hair follicles, and BMPR- I A was de- tected at the tenth day compared with the control group. Results： BMPR- I A expression was observed in the hair follicles of all groups. The average absorbance of BMPR- I A was significantly lower in alopecic area of AGA than in normal area of healthy volunteers（0.223±0.062 vs 0.097±0.038, t=4.39, P〈0.05）, and the average absorbanee of BMPR- I A in occipital scalp of AGA was 0.216±0.051（P〉0.05）. Decreased protein expression of BMPR-I A was also noted in the alopecia group compared with the normal group. Moreover, the gray ratio between BMPR- I A protein and GAPDH in the frontoparietal scalp of AGA patient re- duced from 1.733±0.058 to 0.396±0.036（P〈 0.05）, and the gray ratio of occipital scalp of AGA was 1.574±0.039 with no statistic difference to the control group. The gray ratio of DHT group was 0.367±0.026, which was much lower than 3.441±0.172 of control group（P〈0.05）. RT-PCR results revealed that the mRNA expression level of BMPR- I A of the alopecia group decreased by 47 percent compared with the control group and decreased by 87 percent in the DHT group compared with the control group. Conclusions： The decrease of BMPR- I A is partly induced by DHT, whether the slowdown of the growth and degeneration of hair follicles induced by DHT is due to the the down regulation of BMPR- I A is still to be made clear; and the reduction of BMPR- I A may be associated with ob

关 键 词：骨形成蛋白I型受体A 雄激素源性脱发 器官培养 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]