表没食子儿茶素没食子酸酯对结肠癌细胞株抑制作用的体外研究  

Epigallocatechin-3-gall-ate on Colorectal Cancer Cells in vitro

作　　者：张春霞[1] 王水明[2] 金黑鹰[2] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210029 [2]南京中医药大学第三附属医院全国中医肛肠专科医疗中心,江苏省中西医结合结直肠癌诊疗中心,江苏南京210001

出　　处：《时珍国医国药》2012年第4期833-836,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30572447;30973837)

摘　　要：目的探讨绿茶提取物表没食子儿茶素没食子酸酯(EGCG)对结肠癌细胞株(LoVo细胞、SW480细胞、HT29细胞、HCT-8细胞)增殖的抑制作用及对细胞凋亡和细胞周期的影响,研究其对结肠癌的抑癌机制。方法体外培养结肠癌细胞株,采用不同浓度的EGCG(10,20,35μg/ml)对其进行干预,MTT法检测EGCG对结肠癌细胞株增殖的抑制作用;用流式细胞仪检测EGCG对结肠癌细胞株凋亡和细胞周期的影响。结果 MTT法检测EGCG对结肠癌细胞株的抑制作用均具有浓度依赖性,且HT29细胞株尚具有时间依赖性。流式细胞仪检测EGCG能增强结肠癌细胞株的凋亡率,且剂量与凋亡率呈正相关。EGCG能将SW480细胞和LoVo细胞细胞周期阻滞在G0/G1期,阻碍其向S期转换,将HCT-8细胞阻滞在G2/M期,阻碍其向M期转换,将HT29细胞阻滞在S期,阻碍其向G2期转换,抑制结肠癌细胞株的增殖。结论EGCG对体外培养的结肠癌细胞株的增殖有明显抑制作用,其作用机制与增强细胞凋亡和影响细胞周期有关,具体作用机制有待进一步研究。ObjectiveTo study the effect of epigallocatechin-3-gallate（EGCG） on the proliferation of colorectal cancer cells（LoVo cells 、SW480 cells、HT29cells andHCT-8cells） cell cycle and cell apoptosis. MethodsColorectal cancer cells were cultured in vitro and the proliferation ability of cultured cells in different concentration of EGCG（10,20,35 μg/ml）were tested by MTT analysis,the cell apoptosis and cell cycle were detected with flow cytometry. ResultsThe inhibition of EGCG on colorectal cancer cells showed dose dependence,but showed no time dependence except HT29 cells.EGCG could enhance apoptisis rate of colorectal cancer cells.At the mean time,EGCG could block the cell cycle of SW480 cells and LoVo cells at G0/G1 phase,hind their conversion to S phase;could block HCT-8 cell at G2/M phase,hind their conversion to M phase;could block HT29 cell at S phase,hind their conversion to G2 phase and inhibit their proliferation. ConclusionEGCG can significantly inhibit the proliferation of colorectal cancer cells by changing cell cycle and inducing cell apoptosis.The mechanism may be related to the inhibition to certain gene and signal transduction system.

关 键 词：EGCG 结肠癌细胞株 MTT 细胞凋亡 细胞周期 

分 类 号：R284.2[医药卫生—中药学]