胃肠安对SGC-7901胃癌上皮间质转化相关基因表达影响  

Effects of Wei-Chang-An on the Expression of Epithelial Mesenchymal Transition Related Genes in SGC-7901 Gastric Carcinoma

作　　者：沈克平[1] 王海永[1] 胡兵[1] 刘威[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《时珍国医国药》2012年第4期846-847,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873258);上海市浦东新区中医领军型人才项目(No.PWZ2008-20-104)

摘　　要：目的观察胃肠安对人胃癌SGC-7901裸小鼠皮下移植瘤模型上皮间质转化相关基因表达影响。方法人胃癌细胞SGC-7901建立裸小鼠皮下移植瘤模型,随机分为3组,每组10只。对照组予生理盐水0.3 ml灌胃,1次/d;胃肠安组予胃肠安煎剂0.3 ml灌胃,1次/d;5-Fu组予5-Fu 2 mg腹腔注射,1次/周。免疫组织化学法检测肿瘤组织上皮间质转化相关基因表达情况。结果胃肠安治疗后SGC-7901组织上皮标志基因E-cad表达升高,间质标记基因N-cad、vi-mentin表达降低,磷酸化p38-MAPK、磷酸化TrkA/B、以及Snail蛋白表达降低,差异有统计学意义(P<0.01)。结论胃肠安可抑制SGC-7901胃癌上皮间质转化,可能抑制TrkA/B、p38-MAPK磷酸化以及下调Snail转录因子有关。ObjectiveTo investigate the effects of Wei-Chang-An Decoction,a Chinese herbal formula,on expression of epithelial mesenchymal transition related genes in SGC-7901 gastric carcinoma. MethodsThirty BALB/C nude mice transplanted with SGC-7901 cells were randomly divided into three groups（10 mice per group）,and administered intragastrically with normal saline or Wei-Chang-An Decoction daily,or i.p.administered with 5-FU weekly.Expressions of epithelial mesenchymal transition related genes were detected by immunohistochemistry. ResultsAfter Wei-Chang-An Decoction treatment,expression of E-cad was up-regulated,and expression of N-cad,vimentin,p-p38-MAPK,p-TrkA/B,and Snail were down-regulated compared with control group（P0.01）. ConclusionWei-Chang-An Decoction has inhibitory effects on epithelial mesenchymal transition in gastric carcinoma,and the mechanism may be related to down-regulation of p-p38-MAPK,p-TrkA/B and Snail protein expression.

关 键 词：中药复方 胃肠安 胃癌 上皮间质转化 

分 类 号：R285.5[医药卫生—中药学]