复元活血汤对骨折大鼠转化生长因子β1表达的作用研究  

Effects of Fuyuan Huoxue Tang on Expression of Transforming Growth Factor Beta 1 in Rat Fracture Model

作　　者：于波[1] 张恒[1] 谢进[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250011

出　　处：《时珍国医国药》2012年第4期861-863,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81001529);山东省自然科学基金(No.Q2008C18)

摘　　要：目的观察复元活血汤对骨折SD大鼠生长因子-β1(TGF-β1)mRNA及蛋白表达的影响。方法建立SD大鼠开放性的股骨干骨折髓内固定模型,随机分组设立骨折后3,7,14,21 d 4个时相点。大鼠灌服1 g/ml复元活血汤药液2ml,每天两次。取骨痂组织,通过RT-PCR、ELISA法检测TGF-β1的表达。结果 TGF-β1基因表达在骨折愈合的不同阶段不同,并且高于生理盐水组。结论复元活血汤能刺激骨折SD大鼠TGF-β1 mRNA及蛋白表达,能促进骨折的愈合。ObjectiveTo observe the effects of Fuyuan Huoxue Tang on expression of TGF-β1 mRNA and protein in rat fracture model. MethodsKirschner wires were fixed in the bone marrow of the midshaft femur with the standadzed open fracture in SD rats.The rats were randomly and equally divided into treatment group and saline group,further,each group was assigned into 4 points（3,7,14,21 days after fracture）.The rats in treatment group were administered Fuyuan Huoxue Tang,2ml per day in two administrations.Specimens of bony calluses were collected on the 3 rd,7 th,14 th,21 st days after fracture and the expression of TGF-β1 was detected by RT-PCR and enzyme-linked immunosorbent assay. ResultThe expression of TGF-β1 was increased to the first peak on the 3rd day after fracture,but appeared a low point on the 7th day,and returned to the peak in the second week,then decreased later.The expression of TGF-β1 mRNA was higher than that in saline group. ConclusionFuyuan Huoxue Tang can increase the expression TGF-β1 in bony callus of rats with early fracture,and promote the fracture healing.

关 键 词：元复活血汤 骨折 生长因子-β1 

分 类 号：R285.5[医药卫生—中药学]