异土木香内酯在不同血浆中蛋白结合率的HPLC测定  

Determination of Protein Binding Rate of Isoalantolactone by HPLC

作　　者：张嫚丽[1] 王进[2] 霍长虹[1] 李力更[1] 王于方[1] 史清文[1] 

机构地区：[1]河北医科大学药学院,河北石家庄050017 [2]河北医科大学附属第二医院,河北石家庄050070

出　　处：《时珍国医国药》2012年第4期875-876,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省自然科学基金(No.08B032);河北省留学回国人员科技活动项目(No.200602);河北省卫生厅科研基金(No.08053)

摘　　要：目的应用平衡透析法测定异土木香内酯的蛋白结合率。方法采用HPLC法测定异土木香内酯在大鼠血浆、人血浆和牛血清白蛋白(BSA)中的总浓度及游离药物浓度,并分别计算蛋白结合率。结果结果表明体外异土木香内酯与大鼠血浆、人血浆和牛血清白蛋白(BSA)的蛋白结合率均较高。结论异土木香内酯与血浆蛋白具有较强的结合,临床上与其它药物配伍应用时需注意交互影响。ObjectiveTo determine the protein binding rate of isoalantolactone by the method of equilibrium dialysis. MethodsAn HPLC method was carried out to determine the protein binding rate of isoalantolactone in rat plasma,human plasma and bovine serum albumin（BSA）. ResultsThe protein binding rate of isoalantolactone with the three mediums was high in vitro. ConclusionIsoalantolactone has strong combination with plasma protein,and its interaction effects should be paid attention to when combined with other drugs in clinical use.

关 键 词：异土木香内酯 蛋白结合率 高效液相色谱 

分 类 号：R284[医药卫生—中药学]