鹰嘴豆豆芽降血糖功效成分纯化研究  

Purification of Anti-diabetic Components from Chickpea Sprouts with Polyamide and Macroporous Resin

作　　者：程珍[1] 阿布力米提.伊力 赵海青[1] 马庆玲[1] 高彦华[1] 阿吉艾克拜尔.艾萨 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011

出　　处：《时珍国医国药》2012年第4期913-915,共3页Lishizhen Medicine and Materia Medica Research

基　　金：中国科学院西部行动计划高新技术项目(No.KGCX2-YW-503);国家"973"重点基础研究发展计划项目(No.2009CB526509)

摘　　要：目的研究分离纯化鹰嘴豆豆芽降血糖功效成分的工艺。方法采用聚酰胺-大孔树脂联用技术,考察了HPD100、HPD300、HPD450、HPD600、D101、D141、SA-3、FL-3等8种大孔树脂对鹰嘴豆豆芽降糖活性成分纯化的研究。结果聚酰胺与FL-3大孔树脂联用富集鹰嘴豆豆芽降糖活性成分效果最好,药材与聚酰胺比为5∶1,药材与大孔树脂比为3∶1,树脂径高比为1∶6,以4倍体积的40%乙醇洗除杂,再以70%乙醇洗脱,收集5倍体积洗脱液。富集物降糖活性成分纯度可达52%。结论聚酰胺与FL-3大孔树脂联用富集鹰嘴豆豆芽降血糖功效成分效果最佳,是理想的分离纯化介质。ObjectiveTo develop a polyamide and macroporous resin absorption technique for the purification of crude anti-diabetic components from Cicer arietinum L.spourt. MethodsThe crude anti-diabetic components were subjected to purification with different types of macroporous resins,such as HPD100,HPD300,HPD450,HPD600,D101,D141,SA-3,and FL-3. ResultsIt showed that polyamide combined with FL-3 macroporous resin was the most effective.FL-3 resin was packed by wet method,the quality ratio of FL-3 resin and chickpea sprout was 1：3,the quality ratio of polyamide and chickpea sprout was 1：5,the ratio of diameter to height wass 1∶6.The FL-3 resin was eluted with 5 times volumn 40% ethanol,followed by 5 times 50% ethanol,and the effluents was collected.The purity of anti-diabetic components reached 52%. ConclusionPolyamide and FL-3 resin is an ideal resin with the best enrichment for separating and purifying anti-diabetic components from Cicer arietinum L.spourt.

关 键 词：鹰嘴豆芽 降血糖活性成分 聚酰胺-大孔树脂联用 纯化 

分 类 号：R284.2[医药卫生—中药学]