五种多孔生物陶瓷支架的肌肉植入实验研究  

In vivo study of five porous bioceramic scaffolds implanted in animal muscle

作　　者：许宋锋[1] 王臻[2] 于秀淳[1] 王林[2] 林开利[3] 常江[3] 

机构地区：[1]济南军区总医院骨病科,250031 [2]第四军医大学西京医院骨科,西安 [3]中国科学院上海硅酸盐研究所

出　　处：《中华骨科杂志》2012年第5期507-514,共8页Chinese Journal of Orthopaedics

基　　金：国家973计划资助项目（2005CB522704）;上海市科委资助项目（05DJ14005,07ZR14127）

摘　　要：目的观察多孔生物玻璃（Bioglass）、β-磷酸三钙（β—tricalcium phosphate，β—TCP）、羟基磷灰石（hydroxyapatite，HA）、β-硅酸钙（β—calciumsilicate,β—CS）、α-硅酸钙（α-CS）五种生物陶瓷支架植入家兔肌肉的生物学表现。方法应用添加造孔剂工艺烧结制备得到多孔Bioglass、β—TCP、HA、β—CS和α—CS支架，通过X线衍射、孔隙率测定、生物力学测定进行特征分析。将五种支架植入家兔背部肌肉中，4、8、12、16周取材，通过X线、Micro—CT、组织学、扫描电镜和能谱分析进行观察分析，并取材与支架接触肌肉进行聚合酶链式反应（polymerasechainreaction，PCR）分析骨形态发生蛋白-2（bonemorpho—geneticprotein-2，BMP-2）和BMP-7含量。结果五种支架特征分析表明，抗压强度：Bioglass〉ot—CS〉B—CS〉β—TCP〉HA；弹性模量：α-CS〈β-TCP〈HA〈β-CS〈Bioglass；降解性：β—CS〉α-CS〉β—TCP〉Bioglass〉HA。组织学观察五种支架中均未出现新生骨组织。Bioglass、α-CS、β—CS表面有钙磷层形成，说明具有体内生物活性。16周时，只有β—CS出现BMP-2和BMP-7表达。结论多孔硅酸钙支架具有良好体内生物活性、可降解性，但无体内骨诱导性。Objective To investigate the in vivo biological performance of 5 porous bioceramic scaffolds, which were bioglass, β-tricalcium phosphate （β-TCP）, hydroxyapatite （HA）, β-calcium silicate （β- CS） and α-CS, implanted in rabbit dorsal muscle. Methods The 5 porous bioceramic scaffolds were fabricated by adding pore-forming materials and sintering, and then were investigated by X-ray diffraction, porosity mensuration and biomechanics test. The scaffolds were implanted into rabbit dorsal muscle for 4, 8, 12, 16 weeks, respectively. The samples were analyzed by X-ray, Micro-CT, histological analysis, scanning electron microscope （SEM） and energy dispersive spectrometer （EDS）. The expression of bone morphogenetic protein （BMP-2） and BMP-7 in the muscle in touch with bioceramic scaffolds were also investigated by polymerase chain reaction （PCR）. Results The characteristic analysis of 5 scaffolds showed that the sequence of compressive strength was bioglass〉α-CS〉β-CS〉β-TCP〉HA, the sequence of elasticity modulus wasα-CS〈α-TCP〈HA〈β-CS〈bioglass. It was confirmed by X-ray, Micro-CT and histological analysis that the sequence of biodegradability was β-CS〉α-CS〉β-TCP〉bioglass〉HA. The histological observation showed no new bone formation in five scaffolds. A Ca-P layer was formed in the surface of bioglass, α-CS and β- CS, which suggested their in vivo bioactivity. After 16 weeks, the expression of BMP-2 and BMP-7 was found only in β-CS. Conclusion The porous calcium silicate scaffold, which was promising for bone tissue engineering, was with good in vivo bioactivity and biodegradability, without in vivo osteoinductivity.

关 键 词：硅酸盐类 骨形态发生蛋白质类 兔 

分 类 号：R318.08[医药卫生—生物医学工程]