HPLC测定青海不同海拔野生与栽培羌活中异欧前胡素的含量  

Determination of Isoimperatorin by HPLC in Notoptergium Incisum Colleted from Different Area of Qinghai Province

作　　者：马永贵[1] 王婷[1] 贺玉姣[1] 陈志[1] 

机构地区：[1]青海师范大学青藏高原环境与资源教育部重点实验室,青海西宁810008

出　　处：《青海师范大学学报（自然科学版）》2012年第1期86-88,共3页Journal of Qinghai Normal University(Natural Science Edition)

基　　金：青海省科技厅资助项目"青海道地药材羌活种植技术研究"(2005-N-119)

摘　　要：目的:采用HPLC同时测定青海不同地区羌活中异欧前胡素的含量,为评价羌活质量及人工种植羌活提供理论依据.方法:色谱柱为Kromasil C18柱;流动相:甲醇-0.1%磷酸(70∶30);流速:1.0ml/min;检测波长:310nm.结果:不同产地的羌活异欧前胡素含量有差别,同样产于大通的羌活,人工栽培品种的异欧前胡素含量高于野生品种.结论:羌活中异欧前胡素含量与产地生境、栽培技术和羌活本身品种差异有关.Objective： comparing the content of isoimperatorin by HPLC in Notoptergium Incisum col- leted from different area of Qinghai province, evaluate the quality of Notoptergiurn Incisum and provide evidence for their artificial cultivation in Qinghai. Methods： the method was chromatographed on Kromasil C18column and the mobile phase was methanol --0. 1% phosphoric acid（70 ： 30）. The flow rate was 1.0ml/min and isoimperatorinwere detected at 310 nm. Result： the differences on the content of imperatorin were obvious. The content of isoimperatorin in cultivar is higher than that in wild. Conclusion： the concent of isoimperatorin in Notoptergium Incisum refered to the environmental factors, the cultivation technique and different varieties.

关 键 词：羌活 异欧前胡素 高效液相色谱 

分 类 号：Q949.95[生物学—植物学]