壳聚糖修饰银纳米颗粒的制备及抗菌性能研究  

Preparation and antibacterial properties of chitosan modified silver nanoparticles

作　　者：高敏杰[1] 王志强[2] 孙磊[1] 王治华[3] 赵彦保[1] 

机构地区：[1]河南大学特种功能材料教育部重点实验室,河南开封475004 [2]河南大学物理与电子学院,河南开封475004 [3]河南大学化学化工学院环境和分析化学研究所,河南开封475004

出　　处：《功能材料》2012年第8期1038-1041,共4页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(50701016);中国博士后基金资助项目(2011M500787)

摘　　要：采用液相化学还原法,以壳聚糖为修饰剂,硼氢化钠为还原剂,制备了壳聚糖修饰银纳米颗粒(chitosan-Ag NPs)。通过X射线粉末衍射仪、透射电子显微镜、傅立叶变换红外光谱仪等对所制备样品的结构和形貌进行了表征。结果表明,所制备纳米颗粒具有面心立方Ag的晶型结构,壳聚糖通过氨基和羟基中的N、O原子与Ag+的化学键合作用修饰在纳米颗粒表面,起到了限制颗粒粒径长大和防止其团聚的作用。采用肉汤连续稀释法检测了样品对大肠杆菌和金黄色葡萄球菌的抑菌杀菌性能,结果表明chitosan-Ag NPs具有优异的抗菌性,抗菌性能受到粒径大小的影响。Chitosan modified silver nanoparticles （chitosan-Ag NPs） were synthesized through liquid phase re- duction with sodium borohydride （NaBH4） as the reductant and chitosan as the surface modification agent, re- spectively. The structure and morphology of the as-prepared nanoparticles were investigated by X-ray powder diffraction, transmission electron microscopy, and Fourier transform infrared spectrometry. The results show that the as-synthesized chitosan modified Ag nanoparticles have a face-centered cubic crystalline structure. The surface of Ag nanoparticles is coated by chitosan with the chemical bonds between amino, hydroxyl groups and Ag ions. The antibacterial properties of chitosan-Ag NPs against Escherichia coli （n. coli） and Staphylococcus aureus （S. aureus） were investigated by broth dilution. The results show the as-synthesized chitosan-Ag NPs exhibit good antibacterial properties against E. coli and S. aureus. The grain sizes of nanoparticles have a re- markable effect on the antibacterial properties.

关 键 词：壳聚糖 AG纳米颗粒 表面修饰 肉汤稀释法 抗菌性能 

分 类 号：O648[理学—物理化学]