丹参酮ⅡA增效大鼠骨髓源性内皮祖细胞VEGF、SDF-1表达  

Efficiency of rat bone marrow-derived endothelial progenitor cells on VEGF and SDF-1 expression with the aid of Tanshinone ⅡA

作　　者：杨彦[1] 陈庆伟[1] 曹广煜[1] 李桂琼[1] 

机构地区：[1]重庆医科大学附属第二医院老年心血管病科,重庆400010

出　　处：《激光杂志》2012年第2期83-86,共4页Laser Journal

基　　金：国家自然科学基金面上项目(编号:30970826)

摘　　要：目的:研究在丹参酮ⅡA的辅助下,骨髓源性内皮祖细胞(EPCs)VEGF与SDF-1表达是否能增强,从而促进细胞归巢。方法:Percoll密度梯度离心法分离SD大鼠骨髓单个核细胞,血管内皮生长因子(VEGF)、表皮生长因子((EGF)、碱性成纤维细胞生长因子(bFGF)诱导培养,并进行形态学、免疫学(免疫细胞化学染色)、功能学(Dil-acLDL与FITC-UEA-1双荧光染色)鉴定。将鉴定为EPCs的细胞分为单纯EPCs组(对照组)、EPCs+丹参酮ⅡA组(加药组)。细胞培养第9d,Western blot与实时荧光定量PCR检测各组VEGF、SDF-1基因与蛋白表达。结果:EPCs+丹参酮IIA组VEGF、SDF-1基因与蛋白表达均高于EPCs组(p均<0.05)。结论:丹参酮ⅡA可上调大鼠骨髓源性内皮祖细胞VEGF及SDF-1表达,从而可能在缺血环境中更好促进EPCs归巢,更有效促进血管修复与再生。Objective：To investigate the effect of rat bone marrow-derived endothelial progenitor cells on VEGF expression with the aid of Tanshinone ⅡA.Methods：The mononuclear cells were isolated from rat femoral and tibial bone marrow using percoll density gradient centrifugation,then induced with vascular endothelial growth factor（VEGF）,basic fibroblast growth factor（bFGF） and epidermal growth factor（EGF）for two weeks.The expression of cell markers was assessed by immunocytochemistry,and the attached cells were stained with Dil-acLDL and FITC-UEA-1.Then this study included two groups： EPCs and EPCs＋ Tanshinone ⅡA groups.The expression levels of VEGF and SDF-1 was detected by fluorescent qRT-PCR and western blot.Results：Compared with the EPCs group,EPCs＋ Tanshinone IIA group had a higher VEGF and SDF-1 expression（P0.05）.Conclusions：EPCs can increase VEGF and SDF-1 expression with combination of Tanshinone IIA.Homing rate and repairing efficacy of EPCs can improve also with the aid of Tanshinone IIA.EPCs can increase VEGF and SDF-1 expression in injured myocardium after homing,thus improve angiogenesis efficiently.

关 键 词：骨髓 内皮祖细胞 丹参酮ⅡA 归巢 血管新生 

分 类 号：R541.4[医药卫生—心血管疾病]