离子排斥色谱用于厌氧消化处理的水中脂肪族羧酸的分析(英文)  

Analysis of aliphatic carboxylic acids in anaerobic digestion process waters by ion-exclusion chromatography

作　　者：Kazuaki ITO Jun SAKAMOTO Kazuya NAGAOKA Yohichi TAKAYAMA Takashi KANAHORI Hiroshi SUNAHARA Tsuneo HAYASHI Shinji SATO Takeshi HIROKAWA Kazuhiko TANAKA 

机构地区：[1]Department of Biotechnology and Chemistry,Faculty of Engineering,Kinki University [2]Hokoku Ind.Co.Ltd. [3]Tosoh Co.Ltd. [4]Department of Applied Chemistry,Graduate School of Engineering,Hiroshima University [5]Department of Development Science,Graduate School for International Development and Cooperation,Hiroshima University

出　　处：《色谱》2012年第4期369-373,共5页Chinese Journal of Chromatography

摘　　要：The analysis of seven aliphatic carboxylic acids(formic,acetic,propionic,iso-butyric,n-butyric,iso-valeric and n-valeric acid) in anaerobic digestion process waters for biogas production was examined by ion-exclusion chromatography with dilute acidic eluents(benzoic acid,perfluorobutyric acid(PFBA) and sulfuric acid) and non-suppressed conductivity/ultraviolet(UV) detection.The columns used were a styrene/divinylbenzene-based strongly acidic cation-exchange resin column(TSKgel SCX) and a polymethacrylate-based weakly acidic cation-exchange resin column(TSKgel Super IC-A/C).Good separation was performed on the TSKgel SCX in shorter retention times.For the TSKgel Super IC-A/C,peak shape of the acids was sharp and symmetrical in spite of longer retention times.In addition,the mutual separation of the acids was good except for iso-and n-butyric acids.The better separation and good detection was achieved by using the two columns(TSKgel SCX and TSKgel Super IC-A/C connected in series),lower concentrations of PFBA and sulfuric acid as eluents,non-suppressed conductivity detection and UV detection at 210 nm.This analysis was applied to anaerobic digestion process waters.The chromatograms with conductivity detection were relatively simpler compared with those of UV detection.The use of two columns with different selectivities for the aliphatic carboxylic acids and the two detection modes was effective for the determination and identification of the analytes in anaerobic digestion process waters containing complex matrices.The analysis of seven aliphatic carboxylic acids（formic,acetic,propionic,iso-butyric,n-butyric,iso-valeric and n-valeric acid） in anaerobic digestion process waters for biogas production was examined by ion-exclusion chromatography with dilute acidic eluents（benzoic acid,perfluorobutyric acid（PFBA） and sulfuric acid） and non-suppressed conductivity/ultraviolet（UV） detection.The columns used were a styrene/divinylbenzene-based strongly acidic cation-exchange resin column（TSKgel SCX） and a polymethacrylate-based weakly acidic cation-exchange resin column（TSKgel Super IC-A/C）.Good separation was performed on the TSKgel SCX in shorter retention times.For the TSKgel Super IC-A/C,peak shape of the acids was sharp and symmetrical in spite of longer retention times.In addition,the mutual separation of the acids was good except for iso-and n-butyric acids.The better separation and good detection was achieved by using the two columns（TSKgel SCX and TSKgel Super IC-A/C connected in series）,lower concentrations of PFBA and sulfuric acid as eluents,non-suppressed conductivity detection and UV detection at 210 nm.This analysis was applied to anaerobic digestion process waters.The chromatograms with conductivity detection were relatively simpler compared with those of UV detection.The use of two columns with different selectivities for the aliphatic carboxylic acids and the two detection modes was effective for the determination and identification of the analytes in anaerobic digestion process waters containing complex matrices.

关 键 词：ion-exclusion chromatography(IEC) strongly and weakly acidic cation-exchange resin columns perfluorobutyric and sulfuric acids eluents non-suppressed conductivity and ultraviolet(UV) detections aliphatic carboxylic acids anaerobic digestion process waters 

分 类 号：O658[理学—分析化学]