亲水作用色谱柱与阳离子交换树脂柱结合分离无机离子(英文)  

Ion chromatographic separation of inorganic ions using a combination of hydrophilic interaction chromatographic column and cation-exchange resin column

作　　者：Kaori ARAI Masanobu MORI Takahiro HIRONAGA Hideyuki ITABASHI Kazuhiko TANAKA 

机构地区：[1]Graduate School of Engineering,Gunma University [2]Graduate School for International Development and Cooperation,Hiroshima University

出　　处：《色谱》2012年第4期404-408,共5页Chinese Journal of Chromatography

基　　金：supported by Grant-in-Aid for Scientific Research(23615003)in Japan Society for the Promotion of Science(JSPS)

摘　　要：A combination of hydrophilic interaction chromatographic(HILIC) column and a weakly acidic cation-exchange resin(WCX) column was used for simultaneous separation of inorganic anions and cations by ion chromatography(IC).Firstly,the capability of HILIC column for the separation of analyte ions was evaluated under acidic eluent conditions.The columns used were SeQuant ZIC-HILIC(ZIC-HILIC) with a sulfobetaine-zwitterion stationary phase(ZIC-HILIC) and Acclaim HILIC-10 with a diol stationary phase(HILIC-10).When using tartaric acid as the eluent,the HILIC columns indicated strong retentions for anions,based on ion-pair interaction.Especially,HILIC-10 could strongly retain anions compared with ZIC-HILIC.The selectivity for analyte anions of HILIC-10 with 5 mmol/L tartaric acid eluent was in the order of I-> NO-3 > Br-> Cl-> H2PO-4.However,since HILIC-10 could not separate analyte cations,a WCX column(TSKgel Super IC-A/C) was connected after the HILIC column in series.The combination column system of HILIC and WCX columns could successfully separate ten ions(Na+,NH+4,K+,Mg2+,Ca2+,H2PO-4,Cl-,Br-,NO-3 and I-) with elution of 4 mmol/L tartaric acid plus 8 mmol/L 18-crown-6.The relative standard deviations(RSDs) of analyte ions by the system were in the ranges of 0.02%-0.05% in retention times and 0.18%-5.3% in peak areas through three-time successive injections.The limits of detection at signal-to-noise ratio of 3 were 0.24-0.30 μmol/L for the cations and 0.31-1.2 μmol/L for the anions.This system was applied for the simultaneous determination of the cations and the anions in a vegetable juice sample with satisfactory results.A combination of hydrophilic interaction chromatographic（HILIC） column and a weakly acidic cation-exchange resin（WCX） column was used for simultaneous separation of inorganic anions and cations by ion chromatography（IC）.Firstly,the capability of HILIC column for the separation of analyte ions was evaluated under acidic eluent conditions.The columns used were SeQuant ZIC-HILIC（ZIC-HILIC） with a sulfobetaine-zwitterion stationary phase（ZIC-HILIC） and Acclaim HILIC-10 with a diol stationary phase（HILIC-10）.When using tartaric acid as the eluent,the HILIC columns indicated strong retentions for anions,based on ion-pair interaction.Especially,HILIC-10 could strongly retain anions compared with ZIC-HILIC.The selectivity for analyte anions of HILIC-10 with 5 mmol/L tartaric acid eluent was in the order of I- NO-3 Br- Cl- H2PO-4.However,since HILIC-10 could not separate analyte cations,a WCX column（TSKgel Super IC-A/C） was connected after the HILIC column in series.The combination column system of HILIC and WCX columns could successfully separate ten ions（Na＋,NH＋4,K＋,Mg2＋,Ca2＋,H2PO-4,Cl-,Br-,NO-3 and I-） with elution of 4 mmol/L tartaric acid plus 8 mmol/L 18-crown-6.The relative standard deviations（RSDs） of analyte ions by the system were in the ranges of 0.02%-0.05% in retention times and 0.18%-5.3% in peak areas through three-time successive injections.The limits of detection at signal-to-noise ratio of 3 were 0.24-0.30 μmol/L for the cations and 0.31-1.2 μmol/L for the anions.This system was applied for the simultaneous determination of the cations and the anions in a vegetable juice sample with satisfactory results.

关 键 词：ion chromatography(IC) hydrophilic interaction chromatography(HILIC) CATION-EXCHANGE inorganic ions vegetable sample 

分 类 号：O658[理学—分析化学]