RAPD技术在产气荚膜杆菌基因分型中的应用研究  

Genomic parting analyses of Clostridium perfringens by randomly amplified polymorphic DNA

作　　者：杨明凡[1] 田永军[2] 崔保安[1] 张自富 王学斌[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省畜牧局,河南郑州450003

出　　处：《河南农业大学学报》2000年第1期85-87,共3页Journal of Henan Agricultural University

基　　金："九五"国家科技攻关项目(960030308)

摘　　要：采用随机扩增多态性DNA(RondomlyAmplifiedPolymorphicDNA ,RAPD)技术对产气荚膜杆菌进行PCR扩增。结果产气荚膜杆菌A ,B ,C ,D 4个型均可扩增出约 430bp的条带 ,B ,C型还扩增出约 2 10bp条带 ,B ,D型还扩增出约 12 10bp条带 ,A型还扩增出约 2 40bp和 6 90bp条带 ,各型之间的条带差别明显 ,从而为产气荚膜杆菌的基因分型。RAPD was used to identify the genetypes of Cl.perfringens.The result showed that Cl.perfringens types A,B,C,D all had the 430 bp DNA band.Types B,C also had the 210 bp DNA band.Types B,D also had the 1 210 bp DNA band.Type A had the 240 bp and 690 bp DNA bands.DNA profiles of different serotypes were distinguishable.So RAPD can be used to identify Cl.perfringens serotype and provide basis for immediate PCR diagnosis as a rapid,precise and reliable method.

关 键 词：产气荚膜杆菌 基因 分型 RAPD技术 应用 致病菌 

分 类 号：S852.61[农业科学—基础兽医学]