苏云金杆菌对小地老虎的毒力测定  

Virulence test of Bacillus thuringiensis on black cutworm

作　　者：杨建全[1] 李芳[1] 陈家骅[1] 石全秀[1] 张玉珍[2] 

机构地区：[1]福建农业大学植物保护系,福建福州350002 [2]福建省烟草公司,福建福州350003

出　　处：《福建农业大学学报》2000年第1期65-68,共4页Journal of Fujian Agricultural University

基　　金：福建省烟草公司资助项目!(9711)

摘　　要：采用叶片浸蘸法测定 4株苏云金杆菌 (Bt)菌株对小地老虎 1龄幼虫的毒性 ,毒力回归式分别为 Y =11.6 36 1+2 2 .36 6 5X (Bt94 )、 Y =3.0 74 6 +15.5579X (Bt95)、 Y =8.0 4 0 0 +2 3.52 95X (Bt96 )、 Y =7.340 9+18.2 990 X (Bt97) .Bt对小地老虎 1- 3龄幼虫的毒杀效果随幼虫龄期上升呈显著下降 .Bt处理 3龄幼虫的死亡率仅在 0 - 5.0 0 % ,与对照无显著差异 .经初步观察 ,受测菌株对小地老虎有一定的亚致死效应 ,表现在幼虫对 Bt处理的叶片有拒食现象 。Virulence test of four strains of Bacillus thuringiensis (Bt) on black cutworm [ Agrotis ypsilon (Rottemberg)] was conducted by leaves dipping method. The virulence regression equations were Y=11.6361+ 22.3665X (Bt94), Y=3.0746+15.5579X (Bt95), Y=8.0400+23.5295X (Bt96) and Y=7.3409+ 18.2990X (Bt97), respectively. The virulence decreased sharply with the increase of larvae instar, and the mortality of 3 instar larvae treated with Bt was only 0-5.00%, which was not significantly different from CK. It was also found that Bt had sublethality effect on larvae which were antifeedant to the leaves, and the weights of individuals were lower than those of CK.

关 键 词：苏云金杆菌 小地老虎 毒力测定 生物防治 

分 类 号：S433.82[农业科学—农业昆虫与害虫防治] S476.11[农业科学—植物保护]