生物除磷过程中活性污泥聚磷酶活性的变化  

Variation of Polyphosphate Kinase Activity in Activated Sludge during Biological Phosphorus Removal

作　　者：南亚萍[1] 袁林江[1] 何志仙[1] 王晓昌[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《中国给水排水》2012年第9期26-29,共4页China Water & Wastewater

基　　金：国家自然科学基金资助项目(50878180;51078304)

摘　　要：为提高生物除磷工艺的运行稳定性,采用以A2/O和A/O方式运行的高效除磷反应器,对除磷过程中活性污泥的聚磷酶(PPK)活性、混合液中磷浓度和聚磷颗粒含量进行测定,分析三者之间的关系,探讨PPK活性的变化规律。A2/O及A/O工艺的运行周期均为6 h,运行工况分别为进水/厌氧(1 h)、缺氧(0.5 h)、好氧(1.5 h)、沉淀/排水(1 h)、闲置(2 h)和进水/厌氧(1 h)、好氧(1.5 h)、沉淀/排水(1.5 h)、闲置(2 h)。结果表明:细胞聚磷含量与水中磷浓度的变化均符合典型的生物除磷过程特征,而PPK活性与磷浓度和聚磷含量间并无明显的相关关系,表明PPK并非生物除磷过程的关键限制性酶。In order to improve the operation stability of the biological phosphorus removal process, polyphosphate kinase （PPK） activity in activated sludge, phosphorus concentration and polyphosphate a- mount were determined using a reactor with anaerobic/anoxic/aerobic （A2/O） process and anaerobic/ aerobic （A/O） process. The relations among the PPK activity, the phosphorus concentrations and the polyphosphate amount were analyzed, and variation of PPK activity was investigated. Both of the A2/O and A/O processes were operated with 6 h cycles, The A2/O process consisted of a 1 h filling/anaerobic stage, a 0.5 h anoxic stage, a 1.5 h aerobic stage, a 1 h settling/drawing and a 2 h idle stage. The AZ O process consisted of a 1 h filling/anaerobic stage, a 1.5 h aerobic stage, a 1.5 h settling/drawing and a 2 h idle stage. The results showed that changes in polyphosphate amount and phosphorus concentration were fit with typical characteristics of the biological phosphorus removal process. However, there was no obvious correlation between PPK activity and phosphorus concentration or polyphosphate amount, sugges- ting that PPK is not a key restricting enzyme in the biological phosphorus removal process.

关 键 词：聚磷酶 酶活性 聚磷颗粒 生物除磷 活性污泥 

分 类 号：X703[环境科学与工程—环境工程]