电疗“关元穴”对自然围绝经期大鼠卵巢功能影响的研究  

Effect of Electrical Stimulation of Guanyuan Point on the Ovary of Perimenopausal Rats

作　　者：欧洪琼[1] 方廖琼[1] 翟蓓[1] 白晋[1] 刁庆春[2] 

机构地区：[1]重庆医科大学生物医学工程学院,省部共建超声医学工程国家重点实验室,超声医学工程重庆市市级重点实验室,重庆400016 [2]重庆市第一人民医院皮肤科,重庆400011

出　　处：《中国医科大学学报》2012年第4期300-302,共3页Journal of China Medical University

基　　金：973计划项目(2011CB707900);国家自然科学基金资助项目(30970827)

摘　　要：目的探讨电疗"关元穴"对围绝经期大鼠卵巢功能的影响。方法阴道涂片法筛选围绝经期综合征雌性SD大鼠。选择频率2.5 Hz,强度2 V,连续波,给予大鼠"关元穴"经皮电刺激。采用电化学发光免疫分析法检测大鼠血清雌激素水平,组织学方法观察卵巢组织学结构变化,计数各级卵泡数。结果与对照组比较,电疗"关元穴"(30 min/d或20 min/d),连续刺激10d,能显著提高围绝经期大鼠血清雌激素水平(P<0.05),促进卵巢初级卵泡数量增加(P<0.05)。结论电疗"关元穴"能改变围绝经期综合征大鼠分泌雌激素的功能,影响卵巢卵泡发育。Objective To observe the effect of electrical stimulation of Guanyuan point on the ovary of perimenopausal rats.Methods Female SD rats with perimenopausal syndrome examined by vaginal smear were chosen and given transcutaneous electrical nerve stimulation on Guanyuan point by continuous wave of 2.5 Hz frequency and 2 V intensity.Electrochemiluminescence immunity assay（ECLIA） was applied to detect the estrogen levels in the serum of perimenopausal rats.The changes of ovarian tissue were observed and the number of follicles at all levels was counted by histology.Results The estrogen levels in the serum of perimenopausal rats and the number of ovarian primary follicles were increased with the electrical stimulation on Guanyuan point after 10 days with 30 min/d and 20 min/d compared with the control group（P 〈 0.05）.Conclusion Transcutaneous electrical nerve stimulation on Guanyuan point can increase the secretion function of rats with perimenopausal syndrome and affect the follicular development.

关 键 词：电疗 围绝经期综合征 卵巢组织 阴道涂片 自然围绝经期大鼠 

分 类 号：R445[医药卫生—影像医学与核医学]