COX-2 mRNA在咽喉反流疾病中的表达  

Expression of COX-2 mRNA in Laryngopharyngeal Reflux

作　　者：崔潇[1] 王丽萍[1] 周莹[1] 孙士上[1] 吕艳春[1] 

机构地区：[1]中国医科大学附属盛京医院耳鼻咽喉科,沈阳110004

出　　处：《中国医科大学学报》2012年第4期347-350,共4页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20092135);辽宁省教育厅高校科研计划(2009A757)

摘　　要：目的探索环加氧酶2(COX-2)mRNA在咽喉反流(LPR)疾病病变黏膜组织中的表达情况。方法对临床30例患有声带病变并可疑有咽喉反流的患者行电子鼻咽喉镜及通过反流症状指数量表(RSI)和反流检查计分量表(RFS)进行评分,根据评分划分实验1组(20例),实验2组(10例),取正常黏膜(10例)作为对照组。于手术中取得组织标本,采用Real-time PCR方法,测定各组黏膜中COX-2 mRNA的表达情况。结果 Real time-PCR结果显示:实验1组及实验2组的COX-2 mRNA表达均明显高于正常对照组,差异具有统计学意义(P<0.01,P<0.05);实验1组COX-2 mRNA明显高于实验2组(P<0.05)。结论 COX-2 mRNA在咽喉反流病变黏膜中表达明显升高,表明COX-2参与咽喉反流疾病的发生发展过程,为进一步研究咽喉反流疾病的病理损伤机制、为寻找预防干预方法及药物治疗靶点提供基础。Objective To investigate COX-2 mRNA expression in mucous membrane of patients with laryngopharyngeal reflux（LPR）.Methods Thirty cases with vocal cord lesions and suspected with LPR were examined by electronic nasopharyngeal laryngoscope and scored by the reflux symptom（RSI） and the refluz finding score（RFS）.Based on the scores,they were divided into two groups：experimental group one（20 cases） and experimental group two（10 cases）.Ten cases with normal mucous membrane were used as control group.The mucous membrane samples collected by operations were examined by real-time PCR to determine the expression of COX-2.Results It was showed that the expression of COX-2 mRNA in the two experimental groups was much higher than that in control group,with statistically significant differences（P 〈 0.01,P 〈 0.05）;the expression of COX-2 mRNA in experimental group one was much higher than that in experimental group two,with statistically significant differences（P 〈 0.05）.Conclusion The expression of COX-2 mRNA was much higher in mucous membrane of patients with LPR.It indicates that COX-2 plays a role in the development of LPR.The study can be used as reference for further study of the pathological mechanism,preventive intervention and drug therapy of LPR.

关 键 词：环加氧酶2 咽喉反流 反流症状指数量表 反流检查计分量表 

分 类 号：R767.19[医药卫生—耳鼻咽喉科]