蝉花菌丝对单侧输尿管结扎大鼠肾间质uPA、PAI-1蛋白及mRNA表达的影响  

The Effect of Cordyceps Sobolifera Mycelium on Renal Tubulointerstitial Expression of PAI-1 and UPA in Rat with Unilateral Ureter Obstruction

作　　者：刘玉宁[1] 陈以平[2] 王立红 赵宗江[4] 刘伟敬[5] 李建军[1] 邓跃毅[2] 胡春梅[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]上海中医药大学附属龙华医院,上海200032 [3]北京黎明医院,北京100023 [4]北京中医药大学,北京100029 [5]广东医学院附属医院,湛江524001

出　　处：《中国中西医结合肾病杂志》2012年第3期197-200,共4页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:探讨蝉花菌丝对单侧输尿管结扎大鼠肾间质uPA、PAI-1蛋白及mRNA表达的影响。方法:采用单侧输尿管结扎的方法制备单侧输尿管梗阻(UUO)肾间质纤维化大鼠模型。实验分组为正常组、模型组、假手术组、冬虫夏草组、蝉花高剂量组、蝉花中剂量组和蝉花低剂量组,实验周期为25 d。采用免疫组织化学和原位杂交方法,分别检测肾组织uPA、PAI-1蛋白及其mRNA表达,使用图像分析系统对结果进行处理,采用SPSS11.5统计软件统计分析。结果:模型组肾小管间质中PAI-1蛋白及mRNA的表达均明显高于正常组和假手术组(P<0.01);而uPA蛋白及mRNA的表达则与正常组和假手术组差异无统计学意义(P>0.05);虫草组和不同剂量的蝉花菌丝组的PAI-1蛋白及mRNA表达量明显低于模型组(P<0.05～0.01);而uPA蛋白及mRNA的表达量则明显高于模型组(P<0.05～0.01);蝉花菌丝大剂量组的PAI-1蛋白及mRNA的表达量又较蝉花小剂量组和虫草组为低(P<0.05～0.01)。说明蝉花菌丝能下调UUO大鼠肾小管间质PAI-1蛋白及mRNA的高表达,上调UUO大鼠uPA蛋白及mRNA的低表达,并呈明显的量效依赖关系。结论:本研究表明蝉花菌丝通过调节UUO大鼠肾小管间质uPA/PAI-1蛋白及mRNA的表达紊乱而发挥抗肾间质纤维化的药理作用。Objective：To study the effect of Cordyceps Sobolifera Mycelium（CSM） on renal tubulointerstitial fibrosis through observing the expression of urokinase-type plasminogen activator（uPA） and plasminogen activator inhibitor-1（PAI-1） in rat with obstructive nephropathy.Methods：The rat with interstitial fibrosis induced by unilateral ureter obstruction（UUO） was researched for 25 days.The experimental models were divided into normal control group,sham operation group,UUO group,Cordyceps sinensis（CS）-treated group,high dose CSM（HDCSM）-treated group,middle dose CSM（MDCSM）-treated group and low dose CSM（LDCSM）-treated group.The protein and mRNA expression of uPA and PAI-1 was examined by immunological histochemistry and in situ hybridization technology.Results：The protein and mRNA expression of PAI-1 was significantly up-regulated,although the expression of uPA did not change markedly,in UUO group compared with normal control group and sham operation group（P0.05～0.01）.Treatments with CS and CSM down-regulated the expression of PAI-1（P0.05～0.01） and up-regulated the expression of uPA（P0.05～0.01）.And the expression of PAI-1 was lower in HDCSM-treated group than that in CS-treated group or LDCSM-treated group（P0.05～0.01）.Conclusion：Our study suggests that CSM may ameliorate renal tubulointerstitial fibrosis induced by unilateral ureter obstruction in rat models,which is probably attributable to altering the disturbance of PAI-1 and uPA expression.

关 键 词：蝉花菌丝 纤溶酶原激活物 纤溶酶原激活物抑制物 单侧输尿管结扎 肾间质纤维化 

分 类 号：R285.5[医药卫生—中药学]