两株猪繁殖与呼吸征病毒的分离鉴定及遗传变异分析  

Isolation and Identification of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus and Genetic Variation Analysis

作　　者：张超轶[1] 吴欣伟[1] 闫明菲[1] 张亮权[1] 郭泗虎[1] 黄昌力[1] 黄震[1] 张民泽[1] 赵付荣[1] 张桂红[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国畜牧兽医》2012年第4期82-86,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金会(NSFC)-广东联合基金项目(U0931003);国家生猪现代农业产业技术体系(NYCYTX009)

摘　　要：从广东省发病猪场采集的组织和血清中分离到两株PRRSV,病料经RT-PCR检测为阳性,通过Marc-145细胞进行传代,可产生明显细胞病变,通过间接免疫荧光可检测到荧光信号,两株病毒分别命名为ZH-GD株和ZS-GD株。对两株病毒的ORF5和Nsp2高变区进行序列测定和分析,结果表明,PRRSV ZH-GD株和ZS-GD株的核苷酸序列与欧洲型代表株LV株间的相似性相对较远,与美洲株经典毒株VR-2332间的相似性分别为88.6%和88.1%,与中国经典美洲毒株CH-1a间的相似性分别为94.4%和93.0%。在Nsp2上有30个氨基酸的缺失,与JXA1、XH-GD等高致病性变异株的Nsp2缺失位置一致。分离的两株PRRSV均属于美洲型的变异株PRRSV。Two strains of porcine reproductive and respiratory syndrome virus were isolated from tissue and serum from some Guangdong pig farms and shown PRRS positive in RT-PCR testing by Marc-145.The two strains of virus can passage stably and cause typical cytopathic effect,and fluorescence signal was detected by IFA,were named ZH-GD strain and ZS-GD strain.The analysis of variable region sequences of ORF5 and Nsp2 of two strains of virus showed that,PRRSV ZH-GD strain and ZS-GD strain were far to Europe strain LV,the homology of nuclear nucleotide sequence were 50.9% and 49.2%,and the homology with VR-2332 strain were 88.6% and 88.1%,with classic American CH-1a strain were 94.4 % and 93.0%.And there were 30 amino acids deletion in Nsp2.It shared the same deletion with JXA1,XH-GD and other highly pathogenic variant.Thus,the two strains of PRRSV belonged to variants of American type.

关 键 词：PRRSV 分离鉴定 序列测定和分析 

分 类 号：Q78[生物学—分子生物学]