猪Calsarcin-2基因第1内含子遗传多态性分析  

Genetic Polymorphism Analysis on Intron 1 of Calsarcin-2 in Porcine

作　　者：吴眩[1] 许厚强[1] 乜玉丽[1] 陈祥[1] 张勇[1] 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州省动物遗传育种与繁殖人才基地,贵州贵阳550025

出　　处：《中国畜牧兽医》2012年第4期139-143,共5页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省科技厅条件平台项目<贵州动物转基因研究平台建设>(Z093159);贵州省委组织部人才基地项目<贵州省动物遗传育种与繁殖人才基地>(黔人领发[2009]9号)

摘　　要：以大约克猪、长白猪、杜洛克猪和贵州白香猪杂交及回交的后代为研究对象,根据NCBI公布的序列设计了4对引物,采用测序、PCR-SSCP技术对Calsarcin-2基因第1内含子进行了单核苷酸多态性检测和基因型分析,探讨Calsarcin-2基因第1内含子多态性与肉质性状的关系。结果发现2个SNPs(T613C、C936G),群体遗传学分析表明,在所检测的各多态位点上,不同群体间不同基因型的分布都存在着极显著差异(P<0.01),结合品种特性分析表明,613及936位点所检测的不同基因型和肉质可能具有一定的相关性。In this study,the crossbreed and backcross of Yorkshire,Landrace,Duroc and Guizhou Bai Xiang pig were used to investigate the effect of Calsarcin-2 gene on meat quality.Four pairs of primers for intron 1 of Calsarcin-2 gene were designed according to pig sequences from GenBank.Polymorphisms were detected by PCR-SSCP method.SNPs were detected by DNA sequencing.2 SNPs（T613C,C936G） were identified.Population genetics analysis showed that the distribution of genotypes among five species were extremely significant difference（P0.01）.Association of polymorphisms with breed characteristics indicated that the mutations of 613 and 936 sites may be related to meat quality.

关 键 词：猪 Calsarcin-2基因 肉质 PCR-SSCP 

分 类 号：S828.2[农业科学—畜牧学]