乙型肝炎表面抗原ELISA检测假阴性样本的分析  

Analyses of Samples for False Negative HBsAg with ELISA Assay

作　　者：曹树正[1] 王文武[1] 

机构地区：[1]武汉亚洲心脏病医院检验中心,武汉430022

出　　处：《现代检验医学杂志》2012年第1期9-10,共2页Journal of Modern Laboratory Medicine

摘　　要：目的对酶联免疫吸附试验（ELISA）测定乙型肝炎表面抗原（HBsAg）为假阴性的样本，以微_拄子酶克发光法（MEIA）来检测并进行确认，提供一个处理ELISA检测HBsAg产生假阴性的方法。方法选定武汉亚洲心脏病医院2007年8月～2008年10月ELISA测定乙型肝炎血清学标志物的样本3831例，收集其中单乙型肝炎核心抗体（抗-HBc）阳性和／或乙型肝炎e抗体及抗-HBc同为阳性而HBsAg、己型肝炎表面抗体、乙型肝炎e抗原均为阴性的样本，符合条件的76例样本入选，所有入选的样本均用MEIA法进行HBsAg检测，并对检测为阳性的样本进行抗体中和确认试验确认。结果76例样本中，经MEIA法检测HBsAg为阳性的样本49例，占64．5％；经抗体中和确认试验确认为阳性的样本33例。占43．4％．结论ELISA检测HBsAg弱反应性样本的漏检率很高．需用曼好的检测手段来确认．Objective To test and confirm the samples with microparticle enzyme immunoassay （MEIA） for /alse negative samples of hepatitis B surface antigen （HBsAg） with enzyme linked immunosorbent assay（ELISA）,to provide an approach for ELISA detection of HBsAg produced false negative. Methods There were 3 831 cases of serological markers of HBV with ELISA detection from August 2007 to October 2008 in Wuhan Asia Heart Hospital,among which single anti-HBc and/ or both anti-HBe and anti-HBc were positive,and HBsAg,anti-HBs and HBeAg were all negative samples were selected. There 76 samples were eligible,and all these samples were tested HBsAg with MEIA,and confirmed these positive samples by antibody neutralization confirmatory test. Results Of 76 cases, 49 cases were positive,and 33 eases（43.4%） were confirmed positive by antibody neutralication confirmatory test in the end. Conclusions ELISA has high omission rate for the samples of weak positive HBsAg. Need more good methods to confirm.

关 键 词：乙型肝炎表面抗原 酶联免疫吸附试验 抗体中和确认试验 

分 类 号：R512.62[医药卫生—内科学] R446.61[医药卫生—临床医学]