电针耳甲区不同介入时间对抑郁大鼠行为学的影响  

Effect of Electroacupuncture Stimulation of Auricular Concha Region at Different Time-points on Behavior Changes in Depression Rats

作　　者：刘儒鹏[1] 荣培晶[1] 黄占霞[2] 贲卉[1] 李亮[1] 马慧敏[1] 马迎歌[1] 朱兵[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]北京中医药大学,北京100029

出　　处：《针刺研究》2012年第2期131-135,共5页Acupuncture Research

基　　金：北京市自然科学基金重点项目(7111007);中国中医科学院第四批自主选题(ZZ04002)

摘　　要：目的:观察耳甲部迷走神经区电针刺激对抑郁模型大鼠行为学的干预作用,及不同介入时间的效果差异。方法:80只雄性Wistar大鼠应用开野实验进行筛选,分为模型组(6只)、耳甲电针组(12只)、耳甲预电针组(11只)、非耳甲电针组(12只)。各组大鼠均采用慢性轻度不可预见性复合刺激(CUMS)结合孤养方式造模,并对3个治疗组进行耳甲部电针治疗及非耳甲部电针对照治疗。各组造模前及每个周期结束进行开野实验、糖水摄入实验,评估大鼠行为活动状态。结果:耳甲电针治疗及非耳甲电针治疗对大鼠糖水偏好无影响。耳甲电针组开野实验得分始终高于正常水平;耳甲预电针组造模结束2周后得分下降幅度较大,与造模结束期相比差异有统计学意义(P<0.05);非耳甲电针组造模后及治疗后得分下降极显著(P<0.01)。结论:在持续孤养的条件下,耳甲部迷走神经区电针刺激对CUMS结合孤养模型大鼠行为抑郁状态的发展有一定的抑制作用,耳甲电针组对改善慢性应激模型大鼠在开野实验中活动度的效果好于耳甲预电针组。Objective To observe the effect of electroacupuncture（EA） stimulation of auricular concha region（ACR） on behavior changes of depression rats.Methods A total of 80 male Wistar rats were randomized into depression model（model,n=6）,EA-ACR（n=12）,preventive EA-ACR（Pre-EA-ACR,n=11）and EA-ear-tip（n=12）groups.Depression model was established by chronic unpredictable mild stress for 21 days and isolated breeding.Open-field test and sucrose solution intake prefe-rence test were conducted to assess changes of the rats＇ behavior activities before modeling and on day 22,36 and 50 after star-ting model.For rats of the pre-EA-ACR group,EA（2 Hz,1 mA） was applied to the bilateral auricular concha area for 20 min,once daily for 14 days,beginning from the 8th day on after starting model preparation till the end of the modeling,and for rats of the EA-ACR and EA-ear-tip groups,EA was given to the bilateral ACR and ear-tips respectively,beginning after modeling,once daily for 14 days.Results In comparison with pre-modeling,the percentages of sucrose solution consumption in rats of the model group decreased gradually from day 22 to 50（P0.05）.Compared with the model group,the percentage of sucrose solution consumption in the EA-ACR group was increased significantly on day 50（P0.05）,suggesting an inhibitory effect of EA on the reduction of sucrose intake preference at this time-point.No significant differences were found between the pre-EA-ACR and the model groups and between the EA-ear-tip and model groups as well as between the EA-ACR and model groups at most time-points（P0.05）.Open-field test showed that in comparison with pre-modeling in the same one group,the total scores,crossing and rearing scores on day 22 were decreased considerably in the model and EA-ear-tip groups（P0.05,P0.01）,rather than in the pre-EA-ACR and EA-ARC groups（P0.05）,suggesting a suppressing effect of pre-EA-ACR and EA-ACR on depression behavior.Compared to day 22 in the same one group,the total scores,crossing a

关 键 词：抑郁症 电针 耳甲区 开野实验 糖水消耗实验 

分 类 号：R245.323[医药卫生—针灸推拿学]