黑升麻提取物抗抑郁效应研究  

Study on the Antidepressant-like Effect of Cimicifuga racemosa

作　　者：胡光民[1] 张祖志[1] 彭清和[2] 秦勇[2] 申国明[1] 许浩[2] 

机构地区：[1]安徽中医学院解剖教研室,安徽合肥2300382 [2]安徽中医学院中西医结合研究所,安徽合肥230038

出　　处：《辽宁中医药大学学报》2012年第5期35-38,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：安徽省高校优秀青年基金项目(2009SQRZ116ZD);安徽省人才开发资金项目(2007Z051);安徽中医学院人才引进资金项目(2006rc002);安徽省自然基金资助项目(1208085MC49)

摘　　要：目的:观察黑升麻提取物对去势雌性大鼠强迫游泳行为学的影响,探讨黑升麻提取物的抗抑郁效应及安全性。方法3:0只雌性SD大鼠去势后随即分成去势(OVX)对照组、黑升麻(CR)提取物组和美雌醇(E)组,以雌激素药物美雌醇作对照,观察去势雌性大鼠强迫游泳的不动时间及子宫内膜的变化,放射免疫法测定血浆雌二醇含量,采用免疫组织化学的方法,检测下丘脑室旁核c-fos蛋白的表达。结果:与对照组相比,CR、E均可明显缩短强迫游泳大鼠的不动时间(P<0.01),降低下丘脑室旁核神经元中c-fos的表达(P<0.01);但是E组大鼠血浆雌二醇水平明显升高(P<0.01),子宫内膜显著增生;而CR对去势大鼠血浆雌二醇水平无显著影响(P>0.05),子宫内膜无明显改变。结论:CR可以减少强迫游泳大鼠的不动时间,抑制大鼠的抑郁绝望行为,其抗抑郁效应安全有效。Objective：To study the effect of Cimicifuga racemosa（CR）on the behavior and the expression of c-fos in paraventricular nucleus（PVN）of ovariectomized rats by forced swimming tests（FST）.Method：Sprague Dawley female rats were ovariectomized.After two weeks recovery all rats were administered daily dose of CR and Mestranol for two weeks.The antidepressant effects were evaluated by FST in mice.The levels of E2 in blood plasma were measured by radioimmunoassay.The expression of c-fos was mapped by the immunohistochemcal method.Results：Both CR and Mestranol could significantly suppress the accumulated immobility time of forced swimming（P〈0.01）.The distribution of c-fos in the PVN decreased significantly（P〈0.01）.There is no difference in the levels of E2 in blood plasma between CR group and the OVX group（P〉0.05）.Conclusion：The present study indicates that CR has remarkable antidepressant function.

关 键 词：黑升麻 强迫游泳 去势雌性大鼠 C-FOS 安全性 

分 类 号：R285[医药卫生—中药学]